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1w18
From Proteopedia
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[[Category: glycosyltransferase]] | [[Category: glycosyltransferase]] | ||
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Revision as of 14:16, 30 October 2007
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CRYSTAL STRUCTURE OF LEVANSUCRASE FROM GLUCONACETOBACTER DIAZOTROPHICUS
Overview
The endophytic Gram-negative bacterium Gluconacetobacter diazotrophicus, SRT4 secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10), which converts sucrose into fructooligosaccharides and levan. The enzyme, is included in GH (glycoside hydrolase) family 68 of the sequence-based, classification of glycosidases. The three-dimensional structure of LsdA, has been determined by X-ray crystallography at a resolution of 2.5 A (1, A=0.1 nm). The structure was solved by molecular replacement using the, homologous Bacillus subtilis (Bs) levansucrase (Protein Data Bank, accession code 1OYG) as a search model. LsdA displays a five-bladed, beta-propeller architecture, where the catalytic residues that are, responsible for sucrose hydrolysis are perfectly superimposable with the, ... [(full description)]
About this Structure
1W18 is a [Single protein] structure of sequence from [Gluconacetobacter diazotrophicus] with SO4 as [ligand]. Active as [Levansucrase], with EC number [2.4.1.10]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus., Martinez-Fleites C, Ortiz-Lombardia M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernandez L, Davies GJ, Biochem J. 2005 Aug 15;390(Pt 1):19-27. PMID:15869470
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