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3tsa

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[[Image:3tsa.png|left|200px]]
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==Spinosyn Rhamnosyltransferase SpnG==
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<StructureSection load='3tsa' size='340' side='right' caption='[[3tsa]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3tsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_spinosa Saccharopolyspora spinosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TSA FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spnG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=60894 Saccharopolyspora spinosa])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tsa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tsa RCSB], [http://www.ebi.ac.uk/pdbsum/3tsa PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Spinosyns A and D (spinosad), like many other complex polyketides, are tailored near the end of their biosyntheses through the addition of sugars. SpnG, which catalyzes their 9-OH rhamnosylation, is also capable of adding other monosaccharides to the spinosyn aglycone (AGL) from TDP-sugars; however, the substitution of UDP-d-glucose for TDP-d-glucose as the donor substrate is known to result in a &gt;60000-fold reduction in k(cat). Here, we report the structure of SpnG at 1.65 A resolution, SpnG bound to TDP at 1.86 A resolution, and SpnG bound to AGL at 1.70 A resolution. The SpnG-TDP complex reveals how SpnG employs N202 to discriminate between TDP- and UDP-sugars. A conformational change of several residues in the active site is promoted by the binding of TDP. The SpnG-AGL complex shows that the binding of AGL is mediated via hydrophobic interactions and that H13, the potential catalytic base, is within 3 A of the nucleophilic 9-OH group of AGL. A model for the Michaelis complex was constructed to reveal the features that allow SpnG to transfer diverse sugars; it also revealed that the rhamnosyl moiety is in a skew-boat conformation during the transfer reaction.
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Structural Studies of the Spinosyn Rhamnosyltransferase, SpnG.,Isiorho EA, Liu HW, Keatinge-Clay AT Biochemistry. 2012 Feb 14;51(6):1213-22. Epub 2012 Feb 3. PMID:22283226<ref>PMID:22283226</ref>
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The line below this paragraph, containing "STRUCTURE_3tsa", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3tsa| PDB=3tsa | SCENE= }}
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===Spinosyn Rhamnosyltransferase SpnG===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22283226}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22283226 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22283226}}
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==About this Structure==
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[[3tsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_spinosa Saccharopolyspora spinosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TSA OCA].
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==Reference==
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<ref group="xtra">PMID:022283226</ref><references group="xtra"/>
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[[Category: Saccharopolyspora spinosa]]
[[Category: Saccharopolyspora spinosa]]
[[Category: Isiorho, E A.]]
[[Category: Isiorho, E A.]]

Revision as of 06:18, 5 June 2014

Spinosyn Rhamnosyltransferase SpnG

3tsa, resolution 1.70Å

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