Sandbox Reserved 434
From Proteopedia
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===Additional Features=== | ===Additional Features=== | ||
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| + | <Structure load='1nuv' size='500' frame='true' align='left' caption='The sequence of interest in the enzyme, C40 to C48 bind to the RNA to form a bulge loop.' scene='Insert optional scene name here' /> | ||
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| + | The leadzyme is dependent on the inclusion of lead for catalytic effect: no other ion gives the same result. | ||
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| + | The addition of lanthanide ions has been found to increase the rate of substrate cleavage. | ||
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| + | Studies show that equimolar solutions of neodymium (III) and lead (II) maximize this effect, suggesting that neodymium acts as an acid catalyst. The site at which the RNA is cleaved remains unchanged. | ||
===Credits=== | ===Credits=== | ||
Revision as of 04:31, 5 March 2012
| This Sandbox is Reserved from January 19, 2016, through August 31, 2016 for use for Proteopedia Team Projects by the class Chemistry 423 Biochemistry for Chemists taught by Lynmarie K Thompson at University of Massachusetts Amherst, USA. This reservation includes Sandbox Reserved 425 through Sandbox Reserved 439. |
Contents |
Leadzyme, 1nuv
|
Introduction
Overall Structure
Binding Interactions
In the first half of a two-step reaction, the motif uses Pb2+ to cleave the phosphodiester backbone of the substrate strand, yielding a free 5ʹ′-hydroxyl and a 2ʹ′,3ʹ′-cyclic phosphodiester as products, which is analogous to the activity catalyzed by naturally occurring small ribozymes such as the hammerhead and hairpin. The cyclic phosphate is hydrolyzed subsequently to produce a 3ʹ′-phosphate in a manner similar to that of protein ribonuclease A.
Additional Features
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The leadzyme is dependent on the inclusion of lead for catalytic effect: no other ion gives the same result.
The addition of lanthanide ions has been found to increase the rate of substrate cleavage.
Studies show that equimolar solutions of neodymium (III) and lead (II) maximize this effect, suggesting that neodymium acts as an acid catalyst. The site at which the RNA is cleaved remains unchanged.
Credits
Introduction - name of team member
Overall Structure - name of team member
Drug Binding Site - name of team member
Additional Features - Tom Foley
