1br8
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structure of a binary complex of human antithrombin with a | + | The crystal structure of a binary complex of human antithrombin with a peptide of the same sequence as its reactive loop (P14-P3) has been determined at 2.9 A. The peptide binds as the middle strand s4A in the A beta-sheet, homologously to that of the reactive loop in the latent and cleaved forms of antithrombin. Peptide binding results in the complete expulsion of the hinge region of the loop from the A beta-sheet although the conformation differs from that of heparin-activated antithrombin. The 36-fold increase in the rate of reaction of the binary complex with factor Xa indicates that full loop expulsion alone is not sufficient for complete heparin activation of antithrombin but that this is also dependent on the overall conformation of the molecule. Previous studies have demonstrated that reactive loop peptides can block or reverse the polymerisation of serpins associated with cirrhosis and thrombosis. The antithrombin binary complex structure defines the precise localisation of the blocking peptide in a serpin and provides the basis for rational drug design for mimetics that will prevent polymerisation in vivo and so ameliorate the associated disease. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Abrahams, J | + | [[Category: Abrahams, J P.]] |
| - | [[Category: Carrell, R | + | [[Category: Carrell, R W.]] |
| - | [[Category: Chang, W | + | [[Category: Chang, W S.W.]] |
| - | [[Category: Huntington, J | + | [[Category: Huntington, J A.]] |
[[Category: Jin, L.]] | [[Category: Jin, L.]] | ||
| - | [[Category: Lomas, D | + | [[Category: Lomas, D A.]] |
| - | [[Category: Pei, X | + | [[Category: Pei, X Y.]] |
[[Category: Skinner, R.]] | [[Category: Skinner, R.]] | ||
[[Category: antithrombin]] | [[Category: antithrombin]] | ||
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[[Category: thrombosis]] | [[Category: thrombosis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:14 2008'' |
Revision as of 09:58, 21 February 2008
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IMPLICATIONS FOR FUNCTION AND THERAPY OF A 2.9A STRUCTURE OF BINARY-COMPLEXED ANTITHROMBIN
Overview
The crystal structure of a binary complex of human antithrombin with a peptide of the same sequence as its reactive loop (P14-P3) has been determined at 2.9 A. The peptide binds as the middle strand s4A in the A beta-sheet, homologously to that of the reactive loop in the latent and cleaved forms of antithrombin. Peptide binding results in the complete expulsion of the hinge region of the loop from the A beta-sheet although the conformation differs from that of heparin-activated antithrombin. The 36-fold increase in the rate of reaction of the binary complex with factor Xa indicates that full loop expulsion alone is not sufficient for complete heparin activation of antithrombin but that this is also dependent on the overall conformation of the molecule. Previous studies have demonstrated that reactive loop peptides can block or reverse the polymerisation of serpins associated with cirrhosis and thrombosis. The antithrombin binary complex structure defines the precise localisation of the blocking peptide in a serpin and provides the basis for rational drug design for mimetics that will prevent polymerisation in vivo and so ameliorate the associated disease.
About this Structure
1BR8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Implications for function and therapy of a 2.9 A structure of binary-complexed antithrombin., Skinner R, Chang WS, Jin L, Pei X, Huntington JA, Abrahams JP, Carrell RW, Lomas DA, J Mol Biol. 1998;283(1):9-14. PMID:9761669
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