1cq0
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively) are novel hypothalamic neuropeptides encoded by a single | + | Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively) are novel hypothalamic neuropeptides encoded by a single mRNA transcript; they stimulate food intake. We have determined the three-dimensional solution structure of human hypocretin-2/orexin-B using two-dimensional 1H-NMR data and dynamical simulated annealing calculations. On the basis of NOEs, 3JHNalpha coupling constants and hydrogen-deuterium exchange rates together with chemical shift indices, human hypocretin-2/orexin-B was deduced to consist of two alpha-helices connected with a short linker in both H2O and 30% trifluoroethanol solutions. The helical axis of helix I is oriented about 60-80 degrees relative to helix II. Hybrid distance geometry and simulated-annealing protocols were used to generate an ensemble of 30 structures with no constraint violations greater than 0.03 nm for distances and 3 degrees for angles. In addition, human hypocretin-2/orexin-B shares a similar secondary-structural motif with human neuropeptide Y. This result can form the basis for further study on ligand-receptor recognition of human orexin receptors. |
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bang, E | + | [[Category: Bang, E J.]] |
| - | [[Category: Chae, K | + | [[Category: Chae, K J.]] |
| - | [[Category: Lee, D | + | [[Category: Lee, D W.]] |
| - | [[Category: Lee, K | + | [[Category: Lee, K H.]] |
[[Category: Lee, W.]] | [[Category: Lee, W.]] | ||
[[Category: human hcrt-2/ox-b]] | [[Category: human hcrt-2/ox-b]] | ||
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[[Category: solution structure]] | [[Category: solution structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:08:34 2008'' |
Revision as of 10:08, 21 February 2008
|
SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '
Contents |
Overview
Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively) are novel hypothalamic neuropeptides encoded by a single mRNA transcript; they stimulate food intake. We have determined the three-dimensional solution structure of human hypocretin-2/orexin-B using two-dimensional 1H-NMR data and dynamical simulated annealing calculations. On the basis of NOEs, 3JHNalpha coupling constants and hydrogen-deuterium exchange rates together with chemical shift indices, human hypocretin-2/orexin-B was deduced to consist of two alpha-helices connected with a short linker in both H2O and 30% trifluoroethanol solutions. The helical axis of helix I is oriented about 60-80 degrees relative to helix II. Hybrid distance geometry and simulated-annealing protocols were used to generate an ensemble of 30 structures with no constraint violations greater than 0.03 nm for distances and 3 degrees for angles. In addition, human hypocretin-2/orexin-B shares a similar secondary-structural motif with human neuropeptide Y. This result can form the basis for further study on ligand-receptor recognition of human orexin receptors.
Disease
Known diseases associated with this structure: Narcolepsy OMIM:[602358], Porphyria variegata OMIM:[600923]
About this Structure
1CQ0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of a new hypothalamic neuropeptide, human hypocretin-2/orexin-B., Lee JH, Bang E, Chae KJ, Kim JY, Lee DW, Lee W, Eur J Biochem. 1999 Dec;266(3):831-9. PMID:10583376
Page seeded by OCA on Thu Feb 21 12:08:34 2008
Categories: Homo sapiens | Single protein | Bang, E J. | Chae, K J. | Lee, D W. | Lee, K H. | Lee, W. | Human hcrt-2/ox-b | Neuropeptide | Nmr | Obesity | Solution structure
