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4dww

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[[Image:4dww.jpg|left|200px]]
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==Crystal Structure of Nattokinase from Bacillus subtilis natto==
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<StructureSection load='4dww' size='340' side='right' caption='[[4dww]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4dww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._natto Bacillus subtilis subsp. natto]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DWW FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEB:O-BENZYLSULFONYL-SERINE'>SEB</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dww RCSB], [http://www.ebi.ac.uk/pdbsum/4dww PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nattokinase is a single polypeptide chain composed of 275 amino acids (molecular weight 27,724) which displays strong fibrinolytic activity. Moreover, it can activate other fibrinolytic enzymes such as pro-urokinase and tissue plasminogen activator. In the present study, native nattokinase from Bacillus subtilis natto was purified using gel-filtration chromatography and crystallized to give needle-like crystals which could be used for X-ray diffraction experiments. The crystals belonged to space group C2, with unit-cell parameters a=74.3, b=49.9, c=56.3 A, beta=95.2 degrees . Diffraction images were processed to a resolution of 1.74 A with an Rmerge of 5.2% (15.3% in the highest resolution shell) and a completeness of 69.8% (30.0% in the highest resolution shell). This study reports the first X-ray diffraction analysis of nattokinase.
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Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto.,Yanagisawa Y, Chatake T, Chiba-Kamoshida K, Naito S, Ohsugi T, Sumi H, Yasuda I, Morimoto Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1670-3., Epub 2010 Nov 27. PMID:21139221<ref>PMID:21139221</ref>
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The line below this paragraph, containing "STRUCTURE_4dww", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_4dww| PDB=4dww | SCENE= }}
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===Crystal Structure of Nattokinase from Bacillus subtilis natto===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21139221}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21139221 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21139221}}
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==About this Structure==
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[[4dww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._natto Bacillus subtilis subsp. natto]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWW OCA].
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==Reference==
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<ref group="xtra">PMID:021139221</ref><references group="xtra"/>
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[[Category: Bacillus subtilis subsp. natto]]
[[Category: Bacillus subtilis subsp. natto]]
[[Category: Subtilisin]]
[[Category: Subtilisin]]

Revision as of 07:34, 5 June 2014

Crystal Structure of Nattokinase from Bacillus subtilis natto

4dww, resolution 1.74Å

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