2bvt
From Proteopedia
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
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Revision as of 14:44, 30 October 2007
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THE STRUCTURE OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI EXPLAINS THE PRODUCT SPECIFICITY OF GLYCOSIDE HYDROLASE FAMILY 26 MANNANASES.
Overview
The endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi is a, modular plant cell wall degrading enzyme involved in the hydrolysis of the, backbone of mannan, one of the most abundant polysaccharides of the, hemicellulosic network in the plant cell wall. The crystal structure of a, recombinant truncated endo-beta-1,4-mannanase from C. fimi (CfMan26A-50K), was determined by X-ray crystallography to 2.25 A resolution using the, molecular replacement technique. The overall structure of the enzyme, consists of a core (beta/alpha)8-barrel catalytic module characteristic of, clan GH-A, connected via a linker to an immunoglobulin-like module of, unknown function. A complex with the oligosaccharide mannotriose to 2.9 A, resolution has also been obtained. Both the native structure and ... [(full description)]
About this Structure
2BVT is a [Single protein] structure of sequence from [Cellulomonas fimi] with CAC as [ligand]. Active as [Mannan endo-1,4-beta-mannosidase], with EC number [3.2.1.78]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi., Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L, Biochemistry. 2005 Sep 27;44(38):12700-8. PMID:16171384
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