2c9p
From Proteopedia
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Revision as of 14:57, 30 October 2007
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CU(I)CU(II)-COPC AT PH 4.5
Overview
CopC is a small soluble protein expressed in the periplasm of Pseudomonas, syringae pathovar tomato as part of its copper resistance response (cop, operon). Equilibrium competition reactions confirmed two separated binding, sites with high affinities for Cu(I) (10(-7) > or = K(D) > or = 10(-13) M), and Cu(II) (K(D) = 10(-13(1)) M), respectively. While Cu(I)-CopC was, converted cleanly by O2 to Cu(II)-CopC, the fully loaded form, Cu(I)Cu(II)-CopC was stable in air. Variant forms H1F and H91F exhibited a, lower affinity for Cu(II) than does the wild-type protein while variant, E27G exhibited a higher affinity. Cation exchange chromatography detected, each of the four different types of intermolecular copper transfer, reactions possible between wild type and variant forms: Cu(I) site ... [(full description)]
About this Structure
2C9P is a [Single protein] structure of sequence from [Pseudomonas syringae pv. tomato] with CU and NO3 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Intermolecular transfer of copper ions from the CopC protein of Pseudomonas syringae. Crystal structures of fully loaded Cu(I)Cu(II) forms., Zhang L, Koay M, Maher MJ, Xiao Z, Wedd AG, J Am Chem Soc. 2006 May 3;128(17):5834-50. PMID:16637653
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