This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Journal:JMB:2
From Proteopedia
(Difference between revisions)
| Line 18: | Line 18: | ||
<scene name='Journal:JMB:2/Scene_9_2/1'>TextToBeDisplayed</scene> | <scene name='Journal:JMB:2/Scene_9_2/1'>TextToBeDisplayed</scene> | ||
<scene name='Journal:JMB:2/Scene_10_2/1'>TextToBeDisplayed</scene> | <scene name='Journal:JMB:2/Scene_10_2/1'>TextToBeDisplayed</scene> | ||
| + | <scene name='Journal:JMB:2/Scene_11_2/1'>TextToBeDisplayed</scene> | ||
| + | <scene name='Journal:JMB:2/Scene_12_2/1'>TextToBeDisplayed</scene> | ||
Previously PON1 was <scene name='Journal:JMB:2/Scene_2/2'>solved at 4.5 pH</scene>. We sought a physiologically active pH and <scene name='Journal:JMB:2/Scene_2/3'>solved PON1 at 6.5 pH (overlain with 4.5)</scene>. Note <scene name='Journal:JMB:2/Scene_3/2'>residues 346-348 in the two structures</scene>. Especially, observe the <scene name='Journal:JMB:2/Scene_4/1'>movement of residue 71</scene>. We also solved PON1 at 6.5 pH in <scene name='Journal:JMB:2/Scence_5/3'>complex with 2HQ (a lactone approximate)</scene>. Here, we for the first time observe ordered <scene name='Journal:JMB:2/Scene_6/1'>active site loop density</scene>. The residues colored red <scene name='Journal:JMB:2/Scene_7/1'>contact the active site</scene>. <scene name='Journal:JMB:2/Scene_8/1'>2HQ overlaps with PO4</scene>, suggesting that lactone adopt a similar position. 2HQ makes contact with <scene name='Journal:JMB:2/Scene_9/1'>several catalytic residues</scene>. | Previously PON1 was <scene name='Journal:JMB:2/Scene_2/2'>solved at 4.5 pH</scene>. We sought a physiologically active pH and <scene name='Journal:JMB:2/Scene_2/3'>solved PON1 at 6.5 pH (overlain with 4.5)</scene>. Note <scene name='Journal:JMB:2/Scene_3/2'>residues 346-348 in the two structures</scene>. Especially, observe the <scene name='Journal:JMB:2/Scene_4/1'>movement of residue 71</scene>. We also solved PON1 at 6.5 pH in <scene name='Journal:JMB:2/Scence_5/3'>complex with 2HQ (a lactone approximate)</scene>. Here, we for the first time observe ordered <scene name='Journal:JMB:2/Scene_6/1'>active site loop density</scene>. The residues colored red <scene name='Journal:JMB:2/Scene_7/1'>contact the active site</scene>. <scene name='Journal:JMB:2/Scene_8/1'>2HQ overlaps with PO4</scene>, suggesting that lactone adopt a similar position. 2HQ makes contact with <scene name='Journal:JMB:2/Scene_9/1'>several catalytic residues</scene>. | ||
Revision as of 20:31, 21 March 2012
| |||||||||||
- ↑ Ben-David M, Elias M, Filippi JJ, Dunach E, Silman I, Sussman JL, Tawfik DS. Catalytic Versatility and Backups in Enzyme Active Sites: The Case of Serum Paraoxonase 1. J Mol Biol. 2012 Mar 1. PMID:22387469 doi:10.1016/j.jmb.2012.02.042
Proteopedia Page Contributors and Editors (what is this?)
This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
