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2lj6

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[[Image:2lj6.png|left|200px]]
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==Solution Structure and DNA-binding Properties of the Phosphoesterase Domain of DNA Ligase D==
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<StructureSection load='2lj6' size='340' side='right' caption='[[2lj6]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
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== Structural highlights ==
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[[2lj6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJ6 OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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The phosphoesterase (PE) domain of the bacterial DNA repair enzyme LigD possesses distinctive manganese-dependent 3'-phosphomonoesterase and 3'-phosphodiesterase activities. PE exemplifies a new family of DNA end-healing enzymes found in all phylogenetic domains. Here, we determined the structure of the PE domain of Pseudomonas aeruginosa LigD (PaePE) using solution NMR methodology. PaePE has a disordered N-terminus and a well-folded core that differs in instructive ways from the crystal structure of a PaePE*Mn(2+)* sulfate complex, especially at the active site that is found to be conformationally dynamic. Chemical shift perturbations in the presence of primer-template duplexes with 3'-deoxynucleotide, 3'-deoxynucleotide 3'-phosphate, or 3' ribonucleotide termini reveal the surface used by PaePE to bind substrate DNA and suggest a more efficient engagement in the presence of a 3'-ribonucleotide. Spectral perturbations measured in the presence of weakly catalytic (Cd(2+)) and inhibitory (Zn(2+)) metals provide evidence for significant conformational changes at and near the active site, compared to the relatively modest changes elicited by Mn(2+).
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Solution structure and DNA-binding properties of the phosphoesterase domain of DNA ligase D.,Natarajan A, Dutta K, Temel DB, Nair PA, Shuman S, Ghose R Nucleic Acids Res. 2012 Mar 1;40(5):2076-88. Epub 2011 Nov 13. PMID:22084199<ref>PMID:22084199</ref>
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The line below this paragraph, containing "STRUCTURE_2lj6", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2lj6| PDB=2lj6 | SCENE= }}
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===Solution Structure and DNA-binding Properties of the Phosphoesterase Domain of DNA Ligase D===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22084199}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 22084199 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22084199}}
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==About this Structure==
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[[2lj6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJ6 OCA].
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==Reference==
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<ref group="xtra">PMID:022084199</ref><references group="xtra"/>
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Dutta, K.]]
[[Category: Dutta, K.]]

Revision as of 08:25, 30 April 2014

Solution Structure and DNA-binding Properties of the Phosphoesterase Domain of DNA Ligase D

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