1jfn
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The structure of apo(a) KIVT6 was investigated by two- and | + | The structure of apo(a) KIVT6 was investigated by two- and three-dimensional homo- and heteronuclear NMR spectroscopy. The solution structure of apo(a) KIVT6 contains only a small amount of regular secondary structure elements, comprising a short piece of antiparallel beta-sheet formed by residues Trp62-Tyr64 and Trp72-Tyr74, a short piece of parallel beta-sheet formed by the residues Cys1-Tyr2 and Thr78-Gln79, and a small 3(10)-helix within residues Thr38-Tyr40. The backbone as well as the side chains are arranged in a way similar to those of apo(a) KIVT7, apo(a) KIVT10, and plasminogen K4. We determined additionally the K(d) value of 0.31 +/- 0.04 mM for the binding of epsilon-aminocaproic acid (EACA) to apo(a) KIVT6 and mapped the binding region on apo(a) KIVT6 by means of chemical shift perturbation. This lysine binding activity, which was reported to occur within apo(a) KIVT5-8, is functionally different from the lysine binding activity found for apo(a) KIVT10. |
==Disease== | ==Disease== | ||
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[[Category: Bermel, W.]] | [[Category: Bermel, W.]] | ||
[[Category: Hrzenjak, A.]] | [[Category: Hrzenjak, A.]] | ||
| - | [[Category: Kostner, G | + | [[Category: Kostner, G M.]] |
[[Category: Maderegger, B.]] | [[Category: Maderegger, B.]] | ||
[[Category: Sterk, H.]] | [[Category: Sterk, H.]] | ||
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[[Category: protein-protein recognition]] | [[Category: protein-protein recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:11 2008'' |
Revision as of 11:22, 21 February 2008
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SOLUTION STRUCTURE OF HUMAN APOLIPOPROTEIN(A) KRINGLE IV TYPE 6
Contents |
Overview
The structure of apo(a) KIVT6 was investigated by two- and three-dimensional homo- and heteronuclear NMR spectroscopy. The solution structure of apo(a) KIVT6 contains only a small amount of regular secondary structure elements, comprising a short piece of antiparallel beta-sheet formed by residues Trp62-Tyr64 and Trp72-Tyr74, a short piece of parallel beta-sheet formed by the residues Cys1-Tyr2 and Thr78-Gln79, and a small 3(10)-helix within residues Thr38-Tyr40. The backbone as well as the side chains are arranged in a way similar to those of apo(a) KIVT7, apo(a) KIVT10, and plasminogen K4. We determined additionally the K(d) value of 0.31 +/- 0.04 mM for the binding of epsilon-aminocaproic acid (EACA) to apo(a) KIVT6 and mapped the binding region on apo(a) KIVT6 by means of chemical shift perturbation. This lysine binding activity, which was reported to occur within apo(a) KIVT5-8, is functionally different from the lysine binding activity found for apo(a) KIVT10.
Disease
Known diseases associated with this structure: Coronary artery disease, susceptibility to OMIM:[152200], LPA deficiency, congenital OMIM:[152200]
About this Structure
1JFN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of human apolipoprotein(a) kringle IV type 6., Maderegger B, Bermel W, Hrzenjak A, Kostner GM, Sterk H, Biochemistry. 2002 Jan 15;41(2):660-8. PMID:11781107
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