1k0n
From Proteopedia
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==Overview== | ==Overview== | ||
| - | CLIC1 (NCC27) is a member of the highly conserved class of chloride ion | + | CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exists in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-A resolution. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site. The structure indicates that CLIC1 is likely to be controlled by redox-dependent processes. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bauskin, A | + | [[Category: Bauskin, A R.]] |
| - | [[Category: Breit, S | + | [[Category: Breit, S N.]] |
| - | [[Category: Campbell, T | + | [[Category: Campbell, T J.]] |
| - | [[Category: Curmi, P | + | [[Category: Curmi, P M.G.]] |
| - | [[Category: DeMaere, M | + | [[Category: DeMaere, M Z.]] |
| - | [[Category: Fairlie, W | + | [[Category: Fairlie, W D.]] |
| - | [[Category: Harrop, S | + | [[Category: Harrop, S J.]] |
[[Category: Jankova, L.]] | [[Category: Jankova, L.]] | ||
[[Category: Mazzanti, M.]] | [[Category: Mazzanti, M.]] | ||
[[Category: Pankhurst, S.]] | [[Category: Pankhurst, S.]] | ||
| - | [[Category: Qiu, M | + | [[Category: Qiu, M R.]] |
[[Category: Reztsova, T.]] | [[Category: Reztsova, T.]] | ||
[[Category: Tonini, R.]] | [[Category: Tonini, R.]] | ||
| - | [[Category: Valenzuela, S | + | [[Category: Valenzuela, S M.]] |
[[Category: Warton, K.]] | [[Category: Warton, K.]] | ||
| - | [[Category: Wu, W | + | [[Category: Wu, W M.]] |
[[Category: GTT]] | [[Category: GTT]] | ||
[[Category: chloride ion channel]] | [[Category: chloride ion channel]] | ||
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[[Category: ncc27]] | [[Category: ncc27]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:49 2008'' |
Revision as of 11:28, 21 February 2008
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Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione
Overview
CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exists in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-A resolution. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site. The structure indicates that CLIC1 is likely to be controlled by redox-dependent processes.
About this Structure
1K0N is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution., Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM, J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:11551966
Page seeded by OCA on Thu Feb 21 13:28:49 2008
Categories: Homo sapiens | Single protein | Bauskin, A R. | Breit, S N. | Campbell, T J. | Curmi, P M.G. | DeMaere, M Z. | Fairlie, W D. | Harrop, S J. | Jankova, L. | Mazzanti, M. | Pankhurst, S. | Qiu, M R. | Reztsova, T. | Tonini, R. | Valenzuela, S M. | Warton, K. | Wu, W M. | GTT | Chloride ion channel | Clic1 | Glutathione | Glutathione-s-tranferase superfamily | Ncc27
