1fdv

From Proteopedia

Revision as of 13:07, 30 October 2007

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 MUTANT H221L COMPLEXED WITH NAD+

Overview

Type 1 17beta-hydroxysteroid dehydrogenase (17beta-HSD1), a member of the, short chain dehydrogenase reductase (SDR) family, is responsible for the, synthesis of 17beta-estradiol, the biologically active estrogen involved, in the genesis and development of human breast cancers. Here, we report, the crystal structures of the H221L 17beta-HSD1 mutant complexed to NADP+, and estradiol and the H221L mutant/NAD+ and a H221Q mutant/estradiol, complexes. These structures provide a complete picture of the NADP+-enzyme, interactions involving the flexible 191-199 loop (well ordered in the, H221L mutant) and suggest that the hydrophobic residues Phe192-Met193, could facilitate hydride transfer. 17beta-HSD1 appears to be unique among, the members of the SDR protein family in that one of the two ... [(full description)]

About this Structure

1FDV is a [Single protein] structure of sequence from [Homo sapiens] with SO4 and NAD as [ligands]. Active as [Estradiol 17-beta-dehydrogenase], with EC number [1.1.1.62]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].

Reference

Unusual charge stabilization of NADP+ in 17beta-hydroxysteroid dehydrogenase., Mazza C, Breton R, Housset D, Fontecilla-Camps JC, J Biol Chem. 1998 Apr 3;273(14):8145-52. PMID:9525918

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