1mg4
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The doublecortin-like domains (DCX), which typically occur in tandem, are | + | The doublecortin-like domains (DCX), which typically occur in tandem, are novel microtubule-binding modules. DCX tandems are found in doublecortin, a 360-residue protein expressed in migrating neurons; the doublecortin-like kinase (DCLK); the product of the RP1 gene that is responsible for a form of inherited blindness; and several other proteins. Mutations in the gene encoding doublecortin cause lissencephaly in males and the 'double-cortex syndrome' in females. We here report a solution structure of the N-terminal DCX domain of human doublecortin and a 1.5 A resolution crystal structure of the equivalent domain from human DCLK. Both show a stable, ubiquitin-like tertiary fold with distinct structural similarities to GTPase-binding domains. We also show that the C-terminal DCX domains of both proteins are only partially folded. In functional assays, the N-terminal DCX domain of doublecortin binds only to assembled microtubules, whereas the C-terminal domain binds to both microtubules and unpolymerized tubulin. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bushweller, J | + | [[Category: Bushweller, J H.]] |
[[Category: Cierpickil, T.]] | [[Category: Cierpickil, T.]] | ||
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
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[[Category: Devedjiev, Y.]] | [[Category: Devedjiev, Y.]] | ||
[[Category: Feng, Y.]] | [[Category: Feng, Y.]] | ||
| - | [[Category: Kim, M | + | [[Category: Kim, M H.]] |
[[Category: Krowarsch, D.]] | [[Category: Krowarsch, D.]] | ||
[[Category: Otlewski, J.]] | [[Category: Otlewski, J.]] | ||
| - | [[Category: Walsh, C | + | [[Category: Walsh, C A.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: cortex development]] | [[Category: cortex development]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:08 2008'' |
Revision as of 11:55, 21 February 2008
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STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN
Overview
The doublecortin-like domains (DCX), which typically occur in tandem, are novel microtubule-binding modules. DCX tandems are found in doublecortin, a 360-residue protein expressed in migrating neurons; the doublecortin-like kinase (DCLK); the product of the RP1 gene that is responsible for a form of inherited blindness; and several other proteins. Mutations in the gene encoding doublecortin cause lissencephaly in males and the 'double-cortex syndrome' in females. We here report a solution structure of the N-terminal DCX domain of human doublecortin and a 1.5 A resolution crystal structure of the equivalent domain from human DCLK. Both show a stable, ubiquitin-like tertiary fold with distinct structural similarities to GTPase-binding domains. We also show that the C-terminal DCX domains of both proteins are only partially folded. In functional assays, the N-terminal DCX domain of doublecortin binds only to assembled microtubules, whereas the C-terminal domain binds to both microtubules and unpolymerized tubulin.
About this Structure
1MG4 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
The DCX-domain tandems of doublecortin and doublecortin-like kinase., Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH, Derewenda ZS, Nat Struct Biol. 2003 May;10(5):324-33. PMID:12692530
Page seeded by OCA on Thu Feb 21 13:55:08 2008
Categories: Homo sapiens | Single protein | Bushweller, J H. | Cierpickil, T. | Dauter, Z. | Derewenda, U. | Derewenda, Z. | Devedjiev, Y. | Feng, Y. | Kim, M H. | Krowarsch, D. | Otlewski, J. | Walsh, C A. | SO4 | Cortex development | Dcx domain | Doublecortin-like kinase | Microtubule bundling | X-ray structure
