Sandbox Reserved 458
From Proteopedia
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== Mechanism == | == Mechanism == | ||
[[Image:500px-Creatine kinase rxn.png]] | [[Image:500px-Creatine kinase rxn.png]] | ||
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| + | == Real World Application == | ||
| + | As mention previously, CK is routinely assayed in blood serum samples in a clinical setting. High levels of CK along with other factors such as [[lactate dehydrogenase]] and [[aspartate aminotransferase]] have become reliable indicators of myocardial infarctions. These levels are sometimes drawn in patients that have not experienced clear heart attack symptoms but are at risk for such occurences. | ||
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<ref>McLeish, M. and Kenyon, G. ''Relating Structure to Mechanism in Creatine Kinase'' Critical Reviews in Biochemistry and Molecular Biology, 40:1-20, 2005. [http://dx.doi.org/10.1080/10409230590918577 DOI: 10.1080.10409230590918577]</ref> | <ref>McLeish, M. and Kenyon, G. ''Relating Structure to Mechanism in Creatine Kinase'' Critical Reviews in Biochemistry and Molecular Biology, 40:1-20, 2005. [http://dx.doi.org/10.1080/10409230590918577 DOI: 10.1080.10409230590918577]</ref> | ||
| - | <ref> Knopp, J. ''Knopp's Knotes'' p47, Fifth Edition. | + | <ref> Knopp, J. ''Knopp's Knotes'' p47, Fifth Edition. </ref> |
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| + | <ref> Nienhuis, Mark et. al. ''Prognostic importance of creatine kinase and creatine kinase–MB after primary percutaneous coronary intervention for ST-elevation myocardial infarction'' American Heart Journal, Vol 155, Issue 4, April 2008. pp 673-679 </ref> | ||
Revision as of 19:53, 2 May 2012
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| This Sandbox is Reserved from 13/03/2012, through 01/06/2012 for use in the course "Proteins and Molecular Mechanisms" taught by Robert B. Rose at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 451 through Sandbox Reserved 500. | |||||||
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Creatine KinaseCreatine Kinase (CK), sometimes referred to as Creatine Phosphokinase (CPK), is an enzyme (EC 2.7.3.2). CK is classified as a transferase, which means it facilitates the transfer of a group from one molecule to another. AX + B ---> A + BX The first three numbers of its EC number indicate that it is a phosphotransferase with a nitrogenous group as the acceptor. CK is a very important enzyme for all organisms, as it catalyzes the conversion of creatine into phosphocreatine. Phosphocreatine is used as an energy source for high energy need cells such as smooth muscle cells. CK is clinically relevant in blood serum assays in that an elevated CK level might indicate muscle wasting or myocardial infarction.
StructureThere are four major isozymes in the CK family and have been characterized on the basis of differences in gene and amino acid sequence, as well as tissue localization and immunogenicity. The four isozymes are the muscle (MM-CK), brain (BB-CK), mitochondrial ubiquitous (Miu-CK) and mitochondrial sarcomeric (Mis-CK).
Mechanism
Real World ApplicationAs mention previously, CK is routinely assayed in blood serum samples in a clinical setting. High levels of CK along with other factors such as lactate dehydrogenase and aspartate aminotransferase have become reliable indicators of myocardial infarctions. These levels are sometimes drawn in patients that have not experienced clear heart attack symptoms but are at risk for such occurences.
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