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User:Stephanie Maung/Sandbox
From Proteopedia
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<Structure load='1P4P' size='500' frame='true' align='center' caption='Proteolysed Portion of OspB' scene='User:Stephanie_Maung/Sandbox/Gray_1p4p/1' /> | <Structure load='1P4P' size='500' frame='true' align='center' caption='Proteolysed Portion of OspB' scene='User:Stephanie_Maung/Sandbox/Gray_1p4p/1' /> | ||
| - | <scene name='User:Stephanie_Maung/Sandbox/Gray_1p4p/1'> | + | <scene name='User:Stephanie_Maung/Sandbox/Gray_1p4p/1'>reset</scene> |
| + | |||
| + | H6831 Fab complex binds to a highly accessible region near the C-terminus of OspB, away from the N-terminus lipid anchor. The Fab makes contact at three exposed, adjacent <scene name='User:Stephanie_Maung/Sandbox/Loops_lysine_centered/1'>loops</scene>. The interaction of OspB-Fab complex depends heavily on hydrogen bonding between loops 1,2 and 3. When binding of full-length OspB and Fab fragments of H6831 or CB2 fail, it is usually because lys-253, located on loop 2, has been replaced with a different residue. Studies show that substitutions in basic residues of hen egg-white lysozyme (HEL) that participate in HEL-Fab complexes decreased binding affinity by 400-10,000 times. | ||
Residues 157 - 201 contain the <scene name='User:Stephanie_Maung/Sandbox/1p4p_proteolysed_region_triad/2'>cleaved region</scene> of OspB upon interaction with Fab H6831. | Residues 157 - 201 contain the <scene name='User:Stephanie_Maung/Sandbox/1p4p_proteolysed_region_triad/2'>cleaved region</scene> of OspB upon interaction with Fab H6831. | ||
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| - | <scene name='User:Stephanie_Maung/Sandbox/Loops_lysine_centered/1'>loops 1,2, and 3</scene> | ||
http://proteopedia.org/wiki/index.php/Serine_Proteases | http://proteopedia.org/wiki/index.php/Serine_Proteases | ||
Revision as of 08:35, 3 May 2012
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H6831 Fab complex binds to a highly accessible region near the C-terminus of OspB, away from the N-terminus lipid anchor. The Fab makes contact at three exposed, adjacent . The interaction of OspB-Fab complex depends heavily on hydrogen bonding between loops 1,2 and 3. When binding of full-length OspB and Fab fragments of H6831 or CB2 fail, it is usually because lys-253, located on loop 2, has been replaced with a different residue. Studies show that substitutions in basic residues of hen egg-white lysozyme (HEL) that participate in HEL-Fab complexes decreased binding affinity by 400-10,000 times.
Residues 157 - 201 contain the of OspB upon interaction with Fab H6831.
