1qkl
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The protein hRPABC14.4 is an essential subunit of human RNA polymerases I, II, and III and is required for the transcription of all human nuclear | + | The protein hRPABC14.4 is an essential subunit of human RNA polymerases I, II, and III and is required for the transcription of all human nuclear genes. The structure of hRPABC14.4 was determined by nuclear magnetic resonance spectroscopy. The protein fold comprises a highly conserved central domain forming two antiparallel alpha-helices flanked by the less conserved N- and C-terminal regions forming a five-stranded beta-sandwich. Amino acids from the two helices participate in the generation of a hydrophobic surface area which is conserved in all eukaryotic and archaeal homologous subunits, and likely constitutes a critical macromolecular interaction interface. The hRPABC14.4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Delrio-Portilla, F.]] | [[Category: Delrio-Portilla, F.]] | ||
| - | [[Category: Gaskell, A | + | [[Category: Gaskell, A G.]] |
[[Category: Gilbert, D.]] | [[Category: Gilbert, D.]] | ||
| - | [[Category: Ladias, J | + | [[Category: Ladias, J A.A.]] |
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:47 2008'' |
Revision as of 12:40, 21 February 2008
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HRPABC14.4, ESSENTIAL SUBUNIT OF HUMAN RNA POLYMERASES I, II AND III
Overview
The protein hRPABC14.4 is an essential subunit of human RNA polymerases I, II, and III and is required for the transcription of all human nuclear genes. The structure of hRPABC14.4 was determined by nuclear magnetic resonance spectroscopy. The protein fold comprises a highly conserved central domain forming two antiparallel alpha-helices flanked by the less conserved N- and C-terminal regions forming a five-stranded beta-sandwich. Amino acids from the two helices participate in the generation of a hydrophobic surface area which is conserved in all eukaryotic and archaeal homologous subunits, and likely constitutes a critical macromolecular interaction interface. The hRPABC14.4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases.
About this Structure
1QKL is a Single protein structure of sequence from Homo sapiens. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Solution structure of the hRPABC14.4 subunit of human RNA polymerases., del Rio-Portilla F, Gaskell A, Gilbert D, Ladias JA, Wagner G, Nat Struct Biol. 1999 Nov;6(11):1039-42. PMID:10542096
Page seeded by OCA on Thu Feb 21 14:40:47 2008
