1yfm

From Proteopedia

Revision as of 14:28, 30 October 2007

RECOMBINANT YEAST FUMARASE

Overview

Crystal structures for both native and recombinant forms of yeast fumarase, from Saccharomyces cerevisiae have been completed to moderate resolution, by two separate laboratories. The recombinant form was obtained by the, construction of an expression plasmid for Escherichia coli. Despite a high, level of amino acid sequence similarity, purification of the eukaryotic, enzyme from the wild-type prokaryotic enzyme was feasible. The crystal, structure of the native form, NY-fumarase, encompasses residues R22, through M484, while the recombinant form, RY-fumarase, consists of, residues S27 through L485. Both crystal structures lack the N-terminal, translocation segment. Each subunit of the homo-tetrameric protein has, three domains. The active site is formed by segments from each of three, ... [(full description)]

About this Structure

1YFM is a [Single protein] structure of sequence from [Saccharomyces cerevisiae]. Active as [Fumarate hydratase], with EC number [4.2.1.2]. Structure known Active Site: AVE. Full crystallographic information is available from [OCA].

Reference

Crystal structures of native and recombinant yeast fumarase., Weaver T, Lees M, Zaitsev V, Zaitseva I, Duke E, Lindley P, McSweeny S, Svensson A, Keruchenko J, Keruchenko I, Gladilin K, Banaszak L, J Mol Biol. 1998 Jul 17;280(3):431-42. PMID:9665847

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