4dw3
From Proteopedia
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| - | [[ | + | ==Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with 5'-CMP== |
| + | <StructureSection load='4dw3' size='340' side='right' caption='[[4dw3]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4dw3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_viral_diarrhea_virus_1 Bovine viral diarrhea virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DW3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dvk|4dvk]], [[4dvl|4dvl]], [[4dvn|4dvn]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Erns ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11099 Bovine viral diarrhea virus 1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dw3 RCSB], [http://www.ebi.ac.uk/pdbsum/4dw3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/POLG_BVDVC POLG_BVDVC]] Initial binding to target cell probably involves interaction of E(rns) with glycosaminoglycans. E1 and/or E2 are responsible of cell attachment with CD46 and subsequent fusion after internalization of the virion by endocytosis (By similarity). P7 forms a leader sequence to properly orient NS2 in the membrane (By similarity). Uncleaved NS2-3 is required for production of infectious virus. NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus. NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS4A is a cofactor for the NS3 protease activity (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pestiviruses, which belong to the Flaviviridae family of RNA viruses, are important agents of veterinary diseases causing substantial economical losses in animal farming worldwide. Pestivirus particles display three envelope glycoproteins at their surface: E(rns), E1, and E2. We report here the crystal structure of the catalytic domain of E(rns), the ribonucleolytic activity of which is believed to counteract the innate immunity of the host. The structure reveals a three-dimensional fold corresponding to T2 ribonucleases from plants and fungi. Cocrystallization experiments with mono- and oligonucleotides revealed the structural basis for substrate recognition at two binding sites previously identified for T2 RNases. A detailed analysis of poly-U cleavage products using (31)P-NMR and size exclusion chromatography, together with molecular docking studies, provides a comprehensive mechanistic picture of E(rns) activity on its substrates and reveals the presence of at least one additional nucleotide binding site. | ||
| - | + | Crystal Structure of the Pestivirus Envelope Glycoprotein E(rns) and Mechanistic Analysis of Its Ribonuclease Activity.,Krey T, Bontems F, Vonrhein C, Vaney MC, Bricogne G, Rumenapf T, Rey FA Structure. 2012 May 9;20(5):862-73. PMID:22579253<ref>PMID:22579253</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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| - | == | + | |
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[[Category: Bovine viral diarrhea virus 1]] | [[Category: Bovine viral diarrhea virus 1]] | ||
| - | [[Category: Bontems, F | + | [[Category: Bontems, F]] |
| - | [[Category: Bricogne, G | + | [[Category: Bricogne, G]] |
| - | [[Category: Krey, T | + | [[Category: Krey, T]] |
| - | [[Category: Rey, F A | + | [[Category: Rey, F A]] |
| - | [[Category: Ruemenapf, T | + | [[Category: Ruemenapf, T]] |
| - | [[Category: Vaney, M C | + | [[Category: Vaney, M C]] |
| - | [[Category: Vonrhein, C | + | [[Category: Vonrhein, C]] |
[[Category: T2 ribonuclease]] | [[Category: T2 ribonuclease]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
[[Category: Virus glycoprotein]] | [[Category: Virus glycoprotein]] | ||
Revision as of 21:51, 24 December 2014
Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with 5'-CMP
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