1ulh
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the | + | Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the extracellular region of vascular endothelial cells. The splice variant form (mini TrpRS) functions in vascular endothelial cell apoptosis as an angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA synthetase (TyrRS) functions as an angiogenic cytokine in its truncated form (mini TyrRS). Here, we determined the crystal structure of human mini TrpRS at a resolution of 2.3 A and compared the structure with those of prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA anticodon-binding (TAB) domain insertion, consisting of eight residues in the human TrpRS, abolished the enzyme's apoptotic activity for endothelial cells, whereas its translational catalysis and cell-binding activities remained unchanged. Thus, we have identified the inserted peptide motif that activates the angiostatic signaling. |
==Disease== | ==Disease== | ||
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[[Category: Kim, S.]] | [[Category: Kim, S.]] | ||
[[Category: Kise, Y.]] | [[Category: Kise, Y.]] | ||
| - | [[Category: Lee, S | + | [[Category: Lee, S W.]] |
[[Category: Nureki, O.]] | [[Category: Nureki, O.]] | ||
| - | [[Category: Park, S | + | [[Category: Park, S G.]] |
| - | [[Category: RSGI, RIKEN | + | [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]] |
[[Category: Sengoku, T.]] | [[Category: Sengoku, T.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:47 2008'' |
Revision as of 13:25, 21 February 2008
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A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase
Contents |
Overview
Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the extracellular region of vascular endothelial cells. The splice variant form (mini TrpRS) functions in vascular endothelial cell apoptosis as an angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA synthetase (TyrRS) functions as an angiogenic cytokine in its truncated form (mini TyrRS). Here, we determined the crystal structure of human mini TrpRS at a resolution of 2.3 A and compared the structure with those of prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA anticodon-binding (TAB) domain insertion, consisting of eight residues in the human TrpRS, abolished the enzyme's apoptotic activity for endothelial cells, whereas its translational catalysis and cell-binding activities remained unchanged. Thus, we have identified the inserted peptide motif that activates the angiostatic signaling.
Disease
Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[604032]
About this Structure
1ULH is a Single protein structure of sequence from Homo sapiens. Active as Tryptophan--tRNA ligase, with EC number 6.1.1.2 Full crystallographic information is available from OCA.
Reference
A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase., Kise Y, Lee SW, Park SG, Fukai S, Sengoku T, Ishii R, Yokoyama S, Kim S, Nureki O, Nat Struct Mol Biol. 2004 Feb;11(2):149-56. Epub 2004 Jan 11. PMID:14730354
Page seeded by OCA on Thu Feb 21 15:25:47 2008
Categories: Homo sapiens | Single protein | Tryptophan--tRNA ligase | Ishii, R. | Kim, S. | Kise, Y. | Lee, S W. | Nureki, O. | Park, S G. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Sengoku, T. | Yokoyama, S. | Aminoacylation | Angiostatic cytokine | Apoptosis | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics
