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2kxn

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[[Image:2kxn.png|left|200px]]
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==NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA==
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<StructureSection load='2kxn' size='340' side='right' caption='[[2kxn]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''>
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== Structural highlights ==
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[[2kxn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Tra2-beta1 is a unique splicing factor as its single RNA recognition motif (RRM) is located between two RS (arginine-serine) domains. To understand how this protein recognizes its RNA target, we solved the structure of Tra2-beta1 RRM in complex with RNA. The central 5'-AGAA-3' motif is specifically recognized by residues from the beta-sheet of the RRM and by residues from both extremities flanking the RRM. The structure suggests that RNA binding by Tra2-beta1 induces positioning of the two RS domains relative to one another. By testing the effect of Tra2-beta1 and RNA mutations on the splicing of SMN2 exon 7, we validated the importance of the RNA-protein contacts observed in the structure for the function of Tra2-beta1 and determined the functional sequence of Tra2-beta1 in SMN2 exon 7. Finally, we propose a model for the assembly of multiple RNA binding proteins on this exon.
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Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-beta1.,Clery A, Jayne S, Benderska N, Dominguez C, Stamm S, Allain FH Nat Struct Mol Biol. 2011 Apr;18(4):443-50. Epub 2011 Mar 13. PMID:21399644<ref>PMID:21399644</ref>
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The line below this paragraph, containing "STRUCTURE_2kxn", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2kxn| PDB=2kxn | SCENE= }}
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===NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21399644}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 21399644 is the PubMed ID number.
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{{ABSTRACT_PUBMED_21399644}}
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==About this Structure==
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[[2kxn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA].
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==Reference==
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<ref group="xtra">PMID:021399644</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Allain, F H.T.]]
[[Category: Allain, F H.T.]]

Revision as of 08:29, 30 April 2014

NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA

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