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4dqy

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[[Image:4dqy.jpg|left|200px]]
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==Structure of Human PARP-1 bound to a DNA double strand break==
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<StructureSection load='4dqy' size='340' side='right' caption='[[4dqy]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4dqy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQY FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PARP1, ADPRT, PPOL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dqy RCSB], [http://www.ebi.ac.uk/pdbsum/4dqy PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Poly(ADP-ribose) polymerase-1 (PARP-1) (ADP, adenosine diphosphate) has a modular domain architecture that couples DNA damage detection to poly(ADP-ribosyl)ation activity through a poorly understood mechanism. Here, we report the crystal structure of a DNA double-strand break in complex with human PARP-1 domains essential for activation (Zn1, Zn3, WGR-CAT). PARP-1 engages DNA as a monomer, and the interaction with DNA damage organizes PARP-1 domains into a collapsed conformation that can explain the strong preference for automodification. The Zn1, Zn3, and WGR domains collectively bind to DNA, forming a network of interdomain contacts that links the DNA damage interface to the catalytic domain (CAT). The DNA damage-induced conformation of PARP-1 results in structural distortions that destabilize the CAT. Our results suggest that an increase in CAT protein dynamics underlies the DNA-dependent activation mechanism of PARP-1.
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Structural basis for DNA damage-dependent poly(ADP-ribosyl)ation by human PARP-1.,Langelier MF, Planck JL, Roy S, Pascal JM Science. 2012 May 11;336(6082):728-32. PMID:22582261<ref>PMID:22582261</ref>
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The line below this paragraph, containing "STRUCTURE_4dqy", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_4dqy| PDB=4dqy | SCENE= }}
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===Structure of Human PARP-1 bound to a DNA double strand break===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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<!--
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*[[Poly (ADP-ribose) polymerase|Poly (ADP-ribose) polymerase]]
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The line below this paragraph, {{ABSTRACT_PUBMED_22582261}}, adds the Publication Abstract to the page
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== References ==
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(as it appears on PubMed at http://www.pubmed.gov), where 22582261 is the PubMed ID number.
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<references/>
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__TOC__
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{{ABSTRACT_PUBMED_22582261}}
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</StructureSection>
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==About this Structure==
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[[4dqy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQY OCA].
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==Reference==
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<ref group="xtra">PMID:022582261</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Langelier, M F.]]
[[Category: Langelier, M F.]]

Revision as of 08:27, 5 June 2014

Structure of Human PARP-1 bound to a DNA double strand break

4dqy, resolution 3.25Å

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