2vja
From Proteopedia
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===TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH A NON HYDROLYSABLE SUBSTRATE ANALOGUE, 4-OXO-N,N,N-TRIMETHYLPENTANAMINIUM - ORTHORHOMBIC SPACE GROUP -DATASET A AT 100K=== | ===TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH A NON HYDROLYSABLE SUBSTRATE ANALOGUE, 4-OXO-N,N,N-TRIMETHYLPENTANAMINIUM - ORTHORHOMBIC SPACE GROUP -DATASET A AT 100K=== | ||
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*[[AChE inhibitors and substrates (Part III)|AChE inhibitors and substrates (Part III)]] | *[[AChE inhibitors and substrates (Part III)|AChE inhibitors and substrates (Part III)]] | ||
*[[Acetylcholinesterase|Acetylcholinesterase]] | *[[Acetylcholinesterase|Acetylcholinesterase]] | ||
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==Reference== | ==Reference== | ||
Revision as of 23:12, 26 July 2012
Contents |
TORPEDO CALIFORNICA ACETYLCHOLINESTERASE IN COMPLEX WITH A NON HYDROLYSABLE SUBSTRATE ANALOGUE, 4-OXO-N,N,N-TRIMETHYLPENTANAMINIUM - ORTHORHOMBIC SPACE GROUP -DATASET A AT 100K
Although x-ray crystallography is the most widely used method for macromolecular structure determination, it does not provide dynamical information, and either experimental tricks or complementary experiments must be used to overcome the inherently static nature of crystallographic structures. Here we used specific x-ray damage during temperature-controlled crystallographic experiments at a third-generation synchrotron source to trigger and monitor (Shoot-and-Trap) structural changes putatively involved in an enzymatic reaction. In particular, a nonhydrolyzable substrate analogue of acetylcholinesterase, the "off-switch" at cholinergic synapses, was radiocleaved within the buried enzymatic active site. Subsequent product clearance, observed at 150 K but not at 100 K, indicated exit from the active site possibly via a "backdoor." The simple strategy described here is, in principle, applicable to any enzyme whose structure in complex with a substrate analogue is available and, therefore, could serve as a standard procedure in kinetic crystallography studies.
Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography., Colletier JP, Bourgeois D, Sanson B, Fournier D, Sussman JL, Silman I, Weik M, Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11742-7. Epub 2008 Aug 13. PMID:18701720
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2vja is a 2 chain structure of Acetylcholinesterase with sequence from Torpedo californica. Full crystallographic information is available from OCA.
See Also
Reference
- Colletier JP, Bourgeois D, Sanson B, Fournier D, Sussman JL, Silman I, Weik M. Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11742-7. Epub 2008 Aug 13. PMID:18701720
Categories: Acetylcholinesterase | Torpedo californica | Bourgeois, D. | Colletier, J P. | Fournier, D. | Silman, I. | Sussman, J L. | Weik, M. | Cell junction | Glycoprotein | Gpi-anchor | Hydrolase | Kinetic crystallography | Lipoprotein | Membrane | Neurotransmitter degradation | Serine esterase | Structural dynamic | Substrate analogue | Synapse | Xray damage
