3t50

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[[Image:3t50.jpg|left|200px]]
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==X-ray structure of the LOV domain from the LOV-HK sensory protein from Brucella abortus (dark state).==
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<StructureSection load='3t50' size='340' side='right' caption='[[3t50]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3t50]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brucella_melitensis Brucella melitensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T50 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T50 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BAB2_0652, BMEII0679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29459 Brucella melitensis])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t50 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t50 RCSB], [http://www.ebi.ac.uk/pdbsum/3t50 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Light-oxygen-voltage (LOV) domains are blue-light-activated signaling modules present in a wide range of sensory proteins. Among them, the histidine kinases are the largest group in prokaryotes (LOV-HK). Light modulates the virulence of the pathogenic bacteria Brucella abortus through LOV-HK. One of the striking characteristic of Brucella LOV-HK is the fact that the protein remains activated upon light sensing, without recovering the basal state in the darkness. In contrast, the light state of the isolated LOV domain slowly returns to the dark state. To gain insight into the light activation mechanism, we have characterized by X-ray crystallography and solution NMR spectroscopy the structure of the LOV domain of LOV-HK in the dark state and explored its light-induced conformational changes. The LOV domain adopts the alpha/beta PAS (PER-ARNT-SIM) domain fold and binds the FMN cofactor within a conserved pocket. The domain dimerizes through the hydrophobic beta-scaffold in an antiparallel way. Our results point to the beta-scaffold as a key element in the light activation, validating a conserved structural basis for light-to-signal propagation in LOV proteins.
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The beta-Scaffold of the LOV Domain of the Brucella Light-Activated Histidine Kinase Is a Key Element for Signal Transduction.,Rinaldi J, Gallo M, Klinke S, Paris G, Bonomi HR, Bogomolni RA, Cicero DO, Goldbaum FA J Mol Biol. 2012 Jun 29;420(1-2):112-27. Epub 2012 Apr 11. PMID:22504229<ref>PMID:22504229</ref>
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The line below this paragraph, containing "STRUCTURE_3t50", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_3t50| PDB=3t50 | SCENE= }}
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===X-ray structure of the LOV domain from the LOV-HK sensory protein from Brucella abortus (dark state).===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22504229}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 22504229 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_22504229}}
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==About this Structure==
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[[3t50]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brucella_melitensis Brucella melitensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T50 OCA].
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==Reference==
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<ref group="xtra">PMID:022504229</ref><references group="xtra"/>
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[[Category: Brucella melitensis]]
[[Category: Brucella melitensis]]
[[Category: Histidine kinase]]
[[Category: Histidine kinase]]

Revision as of 06:45, 5 June 2014

X-ray structure of the LOV domain from the LOV-HK sensory protein from Brucella abortus (dark state).

3t50, resolution 1.64Å

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