1a57
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1a57.gif|left|200px]] | + | [[Image:1a57.gif|left|200px]] |
| - | + | ||
| - | '''THE THREE-DIMENSIONAL STRUCTURE OF A HELIX-LESS VARIANT OF INTESTINAL FATTY ACID BINDING PROTEIN, NMR, 20 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1a57 |SIZE=350|CAPTION= <scene name='initialview01'>1a57</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE THREE-DIMENSIONAL STRUCTURE OF A HELIX-LESS VARIANT OF INTESTINAL FATTY ACID BINDING PROTEIN, NMR, 20 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1A57 is a [ | + | 1A57 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A57 OCA]. |
==Reference== | ==Reference== | ||
| - | The three-dimensional structure of a helix-less variant of intestinal fatty acid-binding protein., Steele RA, Emmert DA, Kao J, Hodsdon ME, Frieden C, Cistola DP, Protein Sci. 1998 Jun;7(6):1332-9. PMID:[http:// | + | The three-dimensional structure of a helix-less variant of intestinal fatty acid-binding protein., Steele RA, Emmert DA, Kao J, Hodsdon ME, Frieden C, Cistola DP, Protein Sci. 1998 Jun;7(6):1332-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9655337 9655337] |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: fatty acid-binding]] | [[Category: fatty acid-binding]] | ||
[[Category: lipid transport]] | [[Category: lipid transport]] | ||
| - | [[Category: | + | [[Category: lipocalin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:04 2008'' |
Revision as of 07:53, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE THREE-DIMENSIONAL STRUCTURE OF A HELIX-LESS VARIANT OF INTESTINAL FATTY ACID BINDING PROTEIN, NMR, 20 STRUCTURES
Overview
Intestinal fatty acid-binding protein (I-FABP) is a cytosolic 15.1-kDa protein that appears to function in the intracellular transport and metabolic trafficking of fatty acids. It binds a single molecule of long-chain fatty acid in an enclosed cavity surrounded by two five-stranded antiparallel beta-sheets and a helix-turn-helix domain. To investigate the role of the helical domain, we engineered a variant of I-FABP by deleting 17 contiguous residues and inserting a Ser-Gly linker (Kim K et al., 1996, Biochemistry 35:7553-7558). This variant, termed delta17-SG, was remarkably stable, exhibited a high beta-sheet content and was able to bind fatty acids with some features characteristic of the wild-type protein. In the present study, we determined the structure of the delta17-SG/palmitate complex at atomic resolution using triple-resonance 3D NMR methods. Sequence-specific 1H, 13C, and 15N resonance assignments were established at pH 7.2 and 25 degrees C and used to define the consensus 1H/13C chemical shift-derived secondary structure. Subsequently, an iterative protocol was used to identify 2,544 NOE-derived interproton distance restraints and to calculate its tertiary structure using a unique distance geometry/simulated annealing algorithm. In spite of the sizable deletion, the delta17-SG structure exhibits a backbone conformation that is nearly superimposable with the beta-sheet domain of the wild-type protein. The selective deletion of the alpha-helical domain creates a very large opening that connects the interior ligand-binding cavity with exterior solvent. Unlike wild-type I-FABP, fatty acid dissociation from delta17-SG is structurally and kinetically unimpeded, and a protein conformational transition is not required. The delta17-SG variant of I-FABP is the only wild-type or engineered member of the intracellular lipid-binding protein family whose structure lacks alpha-helices. Thus, delta17-SG I-FABP constitutes a unique model system for investigating the role of the helical domain in ligand-protein recognition, protein stability and folding, lipid transfer mechanisms, and cellular function.
About this Structure
1A57 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of a helix-less variant of intestinal fatty acid-binding protein., Steele RA, Emmert DA, Kao J, Hodsdon ME, Frieden C, Cistola DP, Protein Sci. 1998 Jun;7(6):1332-9. PMID:9655337
Page seeded by OCA on Thu Mar 20 09:53:04 2008
