1a65

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[[Image:1a65.jpg|left|200px]]<br /><applet load="1a65" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1a65.jpg|left|200px]]
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caption="1a65, resolution 2.23&Aring;" />
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'''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS'''<br />
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{{Structure
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|PDB= 1a65 |SIZE=350|CAPTION= <scene name='initialview01'>1a65</scene>, resolution 2.23&Aring;
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|SITE= <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene>
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> and <scene name='pdbligand=O:OXYGEN ATOM'>O</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2]
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|GENE=
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}}
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'''TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1A65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=O:'>O</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] Known structural/functional Sites: <scene name='pdbsite=T1:Type+1+Cu+Binding+Site'>T1</scene> and <scene name='pdbsite=T3:Type+3+Cu+Binding+Site'>T3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A65 OCA].
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1A65 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A65 OCA].
==Reference==
==Reference==
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Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9546223 9546223]
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Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546223 9546223]
[[Category: Coprinopsis cinerea]]
[[Category: Coprinopsis cinerea]]
[[Category: Laccase]]
[[Category: Laccase]]
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[[Category: type-2 copper depleted]]
[[Category: type-2 copper depleted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:30 2008''

Revision as of 07:53, 20 March 2008

Image:1a65.jpg


PDB ID 1a65

Drag the structure with the mouse to rotate
, resolution 2.23Å
Sites: and
Ligands: , , and
Activity: Laccase, with EC number 1.10.3.2
Coordinates: save as pdb, mmCIF, xml



TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS


Overview

Laccase catalyses the oxidation of a variety of organic substrates coupled to the reduction of oxygen to water. It is widely believed to be the simplest representative of the ubiquitous blue multi-copper oxidase family. Laccase is implicated in a wide spectrum of biological activities and, in particular, plays a key role in morphogenesis, development and lignin metabolism in fungi and plants. The structure of laccase from the fungus Coprinus cinereus has been determined by X-ray crystallography at a resolution of 2.2 A. Laccase is a monomer composed of three cupredoxin-like beta-sandwich domains, similar to that found in ascorbate oxidase. In contrast to ascorbate oxidase, however, the mononuclear type-1 Cu site lacks the axial methionine ligand and so exhibits trigonal planar coordination, consistent with its elevated redox potential. Crucially, the structure is trapped in a Cu depleted form in which the putative type-2 Cu atom is completely absent, but in which the remaining type-1 and type-3 Cu sites display full occupancy. Type-2 Cu depletion has unexpected consequences for the coordination of the remaining type-3 Cu atoms.

About this Structure

1A65 is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.

Reference

Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution., Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ, Nat Struct Biol. 1998 Apr;5(4):310-6. PMID:9546223

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