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1a77
From Proteopedia
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| - | [[Image:1a77.jpg|left|200px]] | + | [[Image:1a77.jpg|left|200px]] |
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| - | '''FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII''' | + | {{Structure |
| + | |PDB= 1a77 |SIZE=350|CAPTION= <scene name='initialview01'>1a77</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A77 is a [ | + | 1A77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A77 OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:[http:// | + | The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9699635 9699635] |
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rth]] | [[Category: rth]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:57 2008'' |
Revision as of 07:54, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
Overview
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
About this Structure
1A77 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii., Hwang KY, Baek K, Kim HY, Cho Y, Nat Struct Biol. 1998 Aug;5(8):707-13. PMID:9699635
Page seeded by OCA on Thu Mar 20 09:53:57 2008
