1a8z
From Proteopedia
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| - | [[Image:1a8z.gif|left|200px]] | + | [[Image:1a8z.gif|left|200px]] |
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| - | '''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS''' | + | {{Structure |
| + | |PDB= 1a8z |SIZE=350|CAPTION= <scene name='initialview01'>1a8z</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CU1:COPPER (I) ION'>CU1</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A8Z is a [ | + | 1A8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8Z OCA]. |
==Reference== | ==Reference== | ||
| - | Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:[http:// | + | Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9761859 9761859] |
[[Category: Acidithiobacillus ferrooxidans]] | [[Category: Acidithiobacillus ferrooxidans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: CU1]] | [[Category: CU1]] | ||
[[Category: copper protein]] | [[Category: copper protein]] | ||
| - | [[Category: direct | + | [[Category: direct method]] |
[[Category: electron transport]] | [[Category: electron transport]] | ||
[[Category: medium resolution]] | [[Category: medium resolution]] | ||
[[Category: metalloprotein]] | [[Category: metalloprotein]] | ||
| - | [[Category: | + | [[Category: sa]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:34 2008'' |
Revision as of 07:54, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS
Overview
The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data.
About this Structure
1A8Z is a Single protein structure of sequence from Acidithiobacillus ferrooxidans. Full crystallographic information is available from OCA.
Reference
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:9761859
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