1acw
From Proteopedia
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| - | [[Image:1acw.gif|left|200px]] | + | [[Image:1acw.gif|left|200px]] |
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| - | '''SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1acw |SIZE=350|CAPTION= <scene name='initialview01'>1acw</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ACW is a [ | + | 1ACW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Androctonus_mauretanicus_mauretanicus Androctonus mauretanicus mauretanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ACW OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of P01, a natural scorpion peptide structurally analogous to scorpion toxins specific for apamin-sensitive potassium channel., Blanc E, Fremont V, Sizun P, Meunier S, Van Rietschoten J, Thevand A, Bernassau JM, Darbon H, Proteins. 1996 Mar;24(3):359-69. PMID:[http:// | + | Solution structure of P01, a natural scorpion peptide structurally analogous to scorpion toxins specific for apamin-sensitive potassium channel., Blanc E, Fremont V, Sizun P, Meunier S, Van Rietschoten J, Thevand A, Bernassau JM, Darbon H, Proteins. 1996 Mar;24(3):359-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8778783 8778783] |
[[Category: Androctonus mauretanicus mauretanicus]] | [[Category: Androctonus mauretanicus mauretanicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:55:48 2008'' |
Revision as of 07:55, 20 March 2008
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SOLUTION NMR STRUCTURE OF P01, A NATURAL SCORPION PEPTIDE STRUCTURALLY ANALOGOUS TO SCORPION TOXINS SPECIFIC FOR APAMIN-SENSITIVE POTASSIUM CHANNEL, 25 STRUCTURES
Overview
The venom of the North African scorpion Androctonus mauretanicus mauretanicus possesses numerous highly active neurotoxins that specifically bind to various ion channels. One of these, P05, has been found to bind specifically to calcium-activated potassium channels and also to compete with apamin, a toxin extracted from bee venom. Besides the highly potent ones, several of these peptides (including that of P01) have been purified and been found to possess only a very weak, although significant, activity in competition with apamin. The amino acid sequence of P01 shows that it is shorter than P05 by two residues. This deletion occurs within an alpha-helix stretch (residues 5-12). This alpha-helix has been shown to be involved in the interaction of P05 with its receptor via two arginine residues. These two arginines are absent in the P01 sequence. Furthermore, a proline residue in position 7 of the P01 sequence may act as an alpha-helix breaker. We have determined the solution structure of P01 by conventional two-dimensional 1H nuclear magnetic resonance and show that 1) the proline residue does not disturb the alpha-helix running from residues 5 to 12; 2) the two arginines are topologically replaced by two acidic residues, which explains the drop in activity; 3) the residual binding activity may be due to the histidine residue in position 9; and 4) the overall secondary structure is conserved, i.e., an alpha-helix running from residues 5 to 12, two antiparallel stretches of beta-sheet (residues 15-20 and 23-27) connected by a type I' beta-turn, and three disulfide bridges connecting the alpha-helix to the beta-sheet.
About this Structure
1ACW is a Single protein structure of sequence from Androctonus mauretanicus mauretanicus. Full crystallographic information is available from OCA.
Reference
Solution structure of P01, a natural scorpion peptide structurally analogous to scorpion toxins specific for apamin-sensitive potassium channel., Blanc E, Fremont V, Sizun P, Meunier S, Van Rietschoten J, Thevand A, Bernassau JM, Darbon H, Proteins. 1996 Mar;24(3):359-69. PMID:8778783
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