1aly

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[[Image:1aly.gif|left|200px]]<br /><applet load="1aly" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1aly.gif|left|200px]]
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caption="1aly, resolution 2.00&Aring;" />
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'''CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND'''<br />
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{{Structure
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|PDB= 1aly |SIZE=350|CAPTION= <scene name='initialview01'>1aly</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ALY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ALY OCA].
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1ALY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ALY OCA].
==Reference==
==Reference==
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2 A crystal structure of an extracellular fragment of human CD40 ligand., Karpusas M, Hsu YM, Wang JH, Thompson J, Lederman S, Chess L, Thomas D, Structure. 1995 Oct 15;3(10):1031-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8589998 8589998]
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2 A crystal structure of an extracellular fragment of human CD40 ligand., Karpusas M, Hsu YM, Wang JH, Thompson J, Lederman S, Chess L, Thomas D, Structure. 1995 Oct 15;3(10):1031-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8589998 8589998]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: tnf]]
[[Category: tnf]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:45:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:59:26 2008''

Revision as of 07:59, 20 March 2008


PDB ID 1aly

Drag the structure with the mouse to rotate
, resolution 2.00Å
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND


Contents

Overview

BACKGROUND: The CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family of proteins and is transiently expressed on the surface of activated T cells. The binding of CD40L to CD40, which is expressed on the surface of B cells, provides a critical and unique pathway of cellular activation resulting in antibody isotype switching, regulation of apoptosis, and B cell proliferation and differentiation. Naturally occurring mutations of CD40L result in the clinical hyper-IgM syndrome, characterized by an inability to produce immunoglobulins of the IgG, IgA and IgE isotypes. RESULTS: We have determined the crystal structure of a soluble extracellular fragment of human CD40L to 2 A resolution and with an R factor of 21.8%. Although the molecule forms a trimer similar to that found for other members of the TNF family, such as TNF alpha and lymphotoxin-alpha, and exhibits a similar overall fold, there are considerable differences in several loops including those predicted to be involved in CD40 binding. CONCLUSIONS: The structure suggests that most of the hyper-IgM syndrome mutations affect the folding and stability of the molecule rather than the CD40-binding site directly. Despite the fact that the hyper-IgM syndrome mutations are dispersed in the primary sequence, a large fraction of them are clustered in space in the vicinity of a surface loop, close to the predicted CD40-binding site.

Disease

Known diseases associated with this structure: Immunodeficiency, X-linked, with hyper-IgM OMIM:[300386], Transposition of the great arteries, dextro-looped 1 OMIM:[608771]

About this Structure

1ALY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

2 A crystal structure of an extracellular fragment of human CD40 ligand., Karpusas M, Hsu YM, Wang JH, Thompson J, Lederman S, Chess L, Thomas D, Structure. 1995 Oct 15;3(10):1031-9. PMID:8589998

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