This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1rtu
From Proteopedia
| Line 24: | Line 24: | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:03:46 2007'' |
Revision as of 13:59, 30 October 2007
|
USTILAGO SPHAEROGENA RIBONUCLEASE U2
Overview
The crystal structure of purine-specific ribonuclease (RNase) U2 from, Ustilago sphaerogena has been solved by the molecular replacement methods, using RNase T1 as a search model. The structure, with 114 amino acid, residues, 141 water molecules, and a sulfate ion, is refined to an R, factor of 0.143 at 1.8 A resolution. As evidenced by the electron, densities, residues 49 and 50 are revised to Glu 49 and Asp 50, respectively, and also Asp 45 is identified as a beta-isomerized form to, L-isoaspartate with a beta-peptide linkage. RNase U2 consists of a, beta-hairpin at residues from 7 to 14, a 4.4-turn alpha-helix from 16 to, 32, a central beta-sheet with five strands, and a protruding beta-turn, from 74 to 77. As for the catalytic site residues, His 41, Glu 62, and Arg, 85 are located as ... [(full description)]
About this Structure
1RTU is a [Single protein] structure of sequence from [Ustilago sphaerogena] with SO4 as [ligand]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
Crystal structure of Ustilago sphaerogena ribonuclease U2 at 1.8 A resolution., Noguchi S, Satow Y, Uchida T, Sasaki C, Matsuzaki T, Biochemistry. 1995 Nov 28;34(47):15583-91. PMID:7492561
Page seeded by OCA on Tue Oct 30 16:03:46 2007
