1at5

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[[Image:1at5.gif|left|200px]]<br /><applet load="1at5" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1at5.gif|left|200px]]
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caption="1at5, resolution 1.80&Aring;" />
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'''HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE'''<br />
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{{Structure
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|PDB= 1at5 |SIZE=350|CAPTION= <scene name='initialview01'>1at5</scene>, resolution 1.80&Aring;
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|SITE= <scene name='pdbsite=SUC:Succinimide+Is+Formed+At+ASP+101+-+GLY+102'>SUC</scene>
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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|GENE=
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}}
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'''HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1AT5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=SUC:Succinimide+Is+Formed+At+ASP+101+-+GLY+102'>SUC</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AT5 OCA].
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1AT5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AT5 OCA].
==Reference==
==Reference==
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Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose., Noguchi S, Miyawaki K, Satow Y, J Mol Biol. 1998 Apr 24;278(1):231-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9571046 9571046]
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Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose., Noguchi S, Miyawaki K, Satow Y, J Mol Biol. 1998 Apr 24;278(1):231-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9571046 9571046]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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[[Category: succinimide]]
[[Category: succinimide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:02:02 2008''

Revision as of 08:02, 20 March 2008

Image:1at5.gif


PDB ID 1at5

Drag the structure with the mouse to rotate
, resolution 1.80Å
Sites:
Ligands: and
Activity: Lysozyme, with EC number 3.2.1.17
Coordinates: save as pdb, mmCIF, xml



HEN EGG WHITE LYSOZYME WITH A SUCCINIMIDE RESIDUE


Overview

The isomerization of Asp101 to isoaspartate autocatalytically proceeds via a succinimide intermediate in hen egg-white lysozyme at a mildly acidic condition. The crystal structures of succinimide and isoaspartate forms of the lysozyme proteins, each complexed with a tri-N-acetylchitotriose ligand, have been determined at 1.8 A resolution, and distinctively elucidate coplanar cyclic aminosuccinyl and beta-linked isoaspartyl residues. Compared with the liganded native protein with normal Asp101, succinimide 101 protrudes toward the ligand, and isoaspartate 101 extends away from the ligand. The formations of these residues caused the loss of three hydrogen-bonds between the ligand and the side-chains of Asp101 and Asn103 along with 0.5 A displacement of the ligand location.

About this Structure

1AT5 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose., Noguchi S, Miyawaki K, Satow Y, J Mol Biol. 1998 Apr 24;278(1):231-8. PMID:9571046

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