1auu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1auu.gif|left|200px]]<br /><applet load="1auu" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1auu.gif|left|200px]]
-
caption="1auu" />
+
 
-
'''SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES'''<br />
+
{{Structure
 +
|PDB= 1auu |SIZE=350|CAPTION= <scene name='initialview01'>1auu</scene>
 +
|SITE=
 +
|LIGAND=
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1AUU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUU OCA].
+
1AUU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUU OCA].
==Reference==
==Reference==
-
From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins., Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S, EMBO J. 1997 Aug 15;16(16):5019-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9305643 9305643]
+
From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins., Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S, EMBO J. 1997 Aug 15;16(16):5019-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9305643 9305643]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 18: Line 27:
[[Category: transcription regulation]]
[[Category: transcription regulation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:30 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:02:33 2008''

Revision as of 08:02, 20 March 2008


PDB ID 1auu

Drag the structure with the mouse to rotate
Coordinates: save as pdb, mmCIF, xml



SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES


Overview

SacY is the prototype of a family of regulatory proteins able to prevent transcription termination. It interacts with a 29 nucleotide RNA sequence able to fold into a stem-loop structure and partially overlapping with a terminator sequence located in the 5' leader mRNA region of the gene it controls. We show here that the N-terminal fragment of SacY, SacY(1-55), and the corresponding fragments of other members of the family have antiterminator activities with efficiency and specificity identical to those of the full-length proteins. In vitro, this activity correlates with the specific affinity of SacY(1-55) for its RNA target. UV melting experiments demonstrate that SacY(1-55) binding stabilizes the RNA target structure. The NMR solution structure of SacY(1-55) is very similar to that obtained in the crystal (van Tilbeurgh et al., 1997): the peptide is folded as a symmetrical dimer without any structural homology with other RNA-binding domains yet characterized. According to a preliminary NMR analysis of the SacY(1-55)-RNA complex, the protein dimer is not disrupted upon RNA binding and several residues implicated in RNA recognition are located at the edge of the dimer interface. This suggests a new mode of protein-RNA interaction.

About this Structure

1AUU is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins., Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S, EMBO J. 1997 Aug 15;16(16):5019-29. PMID:9305643

Page seeded by OCA on Thu Mar 20 10:02:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools