1awz
From Proteopedia
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| - | [[Image:1awz.jpg|left|200px]]< | + | [[Image:1awz.jpg|left|200px]] |
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| - | '''3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1awz |SIZE=350|CAPTION= <scene name='initialview01'>1awz</scene> | ||
| + | |SITE= <scene name='pdbsite=NLS:Nucleolar+Localization+Signal'>NLS</scene>, <scene name='pdbsite=RBS:Putative+Cell+Receptor+Binding+Site'>RBS</scene> and <scene name='pdbsite=RIB:Ribonucleolytic+Active+Site'>RIB</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AWZ is a [ | + | 1AWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWZ OCA]. |
==Reference== | ==Reference== | ||
| - | Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:[http:// | + | Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9461294 9461294] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:03:20 2008'' |
Revision as of 08:03, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES
Contents |
Overview
Human angiogenin is a member of the pancreatic ribonuclease superfamily that induces blood vessel formation. Its three-dimensional solution structure has been determined to high resolution by heteronuclear NMR spectroscopy. 30 structures were calculated, based on a total of 1441 assigned NOE correlations, 64 coupling constants and 50 hydrogen bonds. The backbone atomic rms difference from the mean coordinates is 0.067 +/- 0.012 nm and 0.13 nm from the previously determined crystal structure. The side-chain of Gln117 was found to obstruct the active site as observed in the crystal state. There was no evidence of an alternative open form of angiogenin, although two sets of chemical shifts were observed for some residues, mainly around the active site and in the C-terminal segment. The topology of the ribonucleolytic active site is described with a particular emphasis on the conformation and protonation of active-site His residues. The side-chain of His114 adopts two main conformations in solution. In contrast to pancreatic ribonuclease A, His13 was shown to be more basic than His114, with pKa values of 6.65 and 6.05 respectively. The His47 residue is located in an environment very resistant to protonation with a pKa lower than 4.
Disease
Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[105850]
About this Structure
1AWZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:9461294
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