1ayf
From Proteopedia
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| - | [[Image:1ayf.gif|left|200px]] | + | [[Image:1ayf.gif|left|200px]] |
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| - | '''BOVINE ADRENODOXIN (OXIDIZED)''' | + | {{Structure |
| + | |PDB= 1ayf |SIZE=350|CAPTION= <scene name='initialview01'>1ayf</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''BOVINE ADRENODOXIN (OXIDIZED)''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AYF is a [ | + | 1AYF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYF OCA]. |
==Reference== | ==Reference== | ||
| - | New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)., Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U, Structure. 1998 Mar 15;6(3):269-80. PMID:[http:// | + | New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)., Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U, Structure. 1998 Mar 15;6(3):269-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9551550 9551550] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:03:53 2008'' |
Revision as of 08:03, 20 March 2008
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| , resolution 1.85Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BOVINE ADRENODOXIN (OXIDIZED)
Overview
BACKGROUND: Adrenodoxin (Adx) is a [2Fe-2S] ferredoxin involved in steroid hormone biosynthesis in the adrenal gland mitochondrial matrix of mammals. Adx is a small soluble protein that transfers electrons from adrenodoxin reductase (AR) to different cytochrome P450 isoforms where they are consumed in hydroxylation reactions. A crystallographic study of Adx is expected to reveal the structural basis for an important electron transfer reaction mediated by a vertebrate [2Fe-2S] ferredoxin. RESULTS: The crystal structure of a truncated bovine adrenodoxin, Adx(4-108), was determined at 1.85 A resolution and refined to a crystallographic R value of 0.195. The structure was determined using multiple wavelength anomalous dispersion phasing techniques, making use of the iron atoms in the [2Fe-2S] cluster of the protein. The protein displays the compact (alpha + beta) fold typical for [2Fe-2S] ferredoxins. The polypeptide chain is organized into a large core domain and a smaller interaction domain which comprises 35 residues, including all those previously determined to be involved in binding to AR and cytochrome P450. A small interdomain motion is observed as a structural difference between the two independent molecules in the asymmetric unit of the crystal. Charged residues of Adx(4-108) are clustered to yield a strikingly asymmetric electric potential of the protein molecule. CONCLUSIONS: The crystal structure of Adx(4-108) provides the first detailed description of a vertebrate [2Fe-2S] ferredoxin and serves to explain a large body of biochemical studies in terms of a three-dimensional structure. The structure suggests how a change in the redox state of the [2Fe-2S] cluster may be coupled to a domain motion of the protein. It seems likely that the clearly asymmetric charge distribution on the surface of Adx(4-108) and the resulting strong molecular dipole are involved in electrostatic steering of the interactions with AR and cytochrome P450.
About this Structure
1AYF is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)., Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U, Structure. 1998 Mar 15;6(3):269-80. PMID:9551550[[Category: [2fe-2s]ferredoxin]]
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