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1bm8
From Proteopedia
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| - | [[Image:1bm8.jpg|left|200px]] | + | [[Image:1bm8.jpg|left|200px]] |
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| - | '''DNA-BINDING DOMAIN OF MBP1''' | + | {{Structure |
| + | |PDB= 1bm8 |SIZE=350|CAPTION= <scene name='initialview01'>1bm8</scene>, resolution 1.71Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''DNA-BINDING DOMAIN OF MBP1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BM8 is a [ | + | 1BM8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM8 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:[http:// | + | Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9083114 9083114] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Xu, R M.]] | [[Category: Xu, R M.]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
| - | [[Category: | + | [[Category: cyclin]] |
[[Category: dna synthesis]] | [[Category: dna synthesis]] | ||
[[Category: helix-turn-helix dna-binding domain]] | [[Category: helix-turn-helix dna-binding domain]] | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:12:52 2008'' |
Revision as of 08:12, 20 March 2008
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| , resolution 1.71Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA-BINDING DOMAIN OF MBP1
Overview
BACKGROUND: During the cell cycle, cells progress through four distinct phases, G1, S, G2 and M; transcriptional controls play an important role at the transition between these phases. MCB-binding factor (MBF), a transcription factor from budding yeast, binds to the so-called MCB (MluI cell-cycle box) elements found in the promoters of many DNA synthesis genes, and activates the transcription of those at the G1-->S phase transition. MBF is comprised of two proteins, Mbp1 and Swi6. RESULTS: The three-dimensional structure of the N-terminal DNA-binding domain of Mbp1 has been determined by multiwavelength anomalous diffraction from crystals of the selenomethionyl variant of the protein. The structure is composed of a six-stranded beta sheet interspersed with two pairs of alpha helices. The most conserved core region among Mbp1-related transcription factors folds into a central helix-turn-helix motif with a short N-terminal beta strand and a C-terminal beta hairpin. CONCLUSIONS: Despite little sequence similarity, the structure within the core region of the Mbp1 N-terminal domain exhibits a similar fold to that of the DNA-binding domains of other proteins, such as hepatocyte nuclear factor-3gamma and histone H5 from eukaryotes, and the prokaryotic catabolite gene activator. However, the structure outside the core region defines Mbp1 as a larger entity with substructures that stabilize and display the helix-turn-helix motif.
About this Structure
1BM8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:9083114
Page seeded by OCA on Thu Mar 20 10:12:52 2008
