1bn8
From Proteopedia
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| - | [[Image:1bn8.jpg|left|200px]] | + | [[Image:1bn8.jpg|left|200px]] |
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| - | '''BACILLUS SUBTILIS PECTATE LYASE''' | + | {{Structure |
| + | |PDB= 1bn8 |SIZE=350|CAPTION= <scene name='initialview01'>1bn8</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''BACILLUS SUBTILIS PECTATE LYASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BN8 is a [ | + | 1BN8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BN8 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of Bacillus subtilis pectate lyase in complex with calcium., Pickersgill R, Jenkins J, Harris G, Nasser W, Robert-Baudouy J, Nat Struct Biol. 1994 Oct;1(10):717-23. PMID:[http:// | + | The structure of Bacillus subtilis pectate lyase in complex with calcium., Pickersgill R, Jenkins J, Harris G, Nasser W, Robert-Baudouy J, Nat Struct Biol. 1994 Oct;1(10):717-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7634076 7634076] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Pectate lyase]] | [[Category: Pectate lyase]] | ||
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[[Category: parallel beta-helix]] | [[Category: parallel beta-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:13:16 2008'' |
Revision as of 08:13, 20 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Pectate lyase, with EC number 4.2.2.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BACILLUS SUBTILIS PECTATE LYASE
Overview
We have solved the structure of the Bacillus subtilis pectate lyase (BsPel) in complex with calcium. The structure consists of a parallel beta-helix domain and a loop region. The alpha L-bounded beta-strand seen in BsPel is a new element of protein structure and its frequent occurrence suggests it is an important characteristic of the parallel beta-helix. A pronounced cleft is formed between the loops and the parallel beta-helix domain and we propose that this is the active site cleft. Calcium, essential for the activity of the enzyme, binds at the bottom of this cleft and an arginine residue close to the calcium, which is conserved across all pectin and pectate lyases, may be involved in catalysis.
About this Structure
1BN8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
The structure of Bacillus subtilis pectate lyase in complex with calcium., Pickersgill R, Jenkins J, Harris G, Nasser W, Robert-Baudouy J, Nat Struct Biol. 1994 Oct;1(10):717-23. PMID:7634076
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