1bqr
From Proteopedia
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| - | [[Image:1bqr.gif|left|200px]] | + | [[Image:1bqr.gif|left|200px]] |
| - | + | ||
| - | '''REDUCED PSEUDOAZURIN''' | + | {{Structure |
| + | |PDB= 1bqr |SIZE=350|CAPTION= <scene name='initialview01'>1bqr</scene>, resolution 1.6Å | ||
| + | |SITE= <scene name='pdbsite=COP:Reduced+Cu+Coordination'>COP</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''REDUCED PSEUDOAZURIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BQR is a [ | + | 1BQR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQR OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine., Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y, J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:[http:// | + | Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine., Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y, J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10364229 10364229] |
[[Category: Achromobacter cycloclastes]] | [[Category: Achromobacter cycloclastes]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:14:35 2008'' |
Revision as of 08:14, 20 March 2008
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| , resolution 1.6Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
REDUCED PSEUDOAZURIN
Overview
The crystal structures of oxidized and reduced pseudoazurins from a denitrifying bacterium, Achromobacter cycloclastes IAM1013, have been determined at 1.35- and 1.6-A resolutions, respectively. The copper site in the oxidized state exhibits a distorted tetrahedral structure like those of other pseudoazurins. However, not only a small change of the copper geometry, but concerted peptide bond flips are identified. The imidazole ring of remote His6 has a hydrogen bonding distance of 2.73 A between N-delta1(His6) and O-gamma1(Thr36) in the oxidized protein. When the protein is reduced at pH 6.0, the imidazole ring rotates by 30.3 degrees and moves 1.00 A away from the position of the oxidized state. A new hydrogen bond between N-epsilon2(His6) and O-epsilon1(Glu4) is formed with a distance of 3.03 A, while the hydrogen bond between N-delta1(His6)-O-gamma1(Thr36) is maintained with an interatomic distance of 2.81 A. A concomitant peptide bond flip of main chain between Ile34 and Thr36 occurs.
About this Structure
1BQR is a Single protein structure of sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA.
Reference
Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine., Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y, J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:10364229
Page seeded by OCA on Thu Mar 20 10:14:35 2008
