1btc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1btc.gif|left|200px]]<br /><applet load="1btc" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1btc.gif|left|200px]]
-
caption="1btc, resolution 2.0&Aring;" />
+
 
-
'''THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN'''<br />
+
{{Structure
 +
|PDB= 1btc |SIZE=350|CAPTION= <scene name='initialview01'>1btc</scene>, resolution 2.0&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2]
 +
|GENE=
 +
}}
 +
 
 +
'''THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1BTC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-amylase Beta-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.2 3.2.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTC OCA].
+
1BTC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BTC OCA].
==Reference==
==Reference==
-
Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem. 1992 Oct;112(4):541-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1491009 1491009]
+
Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem. 1992 Oct;112(4):541-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1491009 1491009]
[[Category: Beta-amylase]]
[[Category: Beta-amylase]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 24: Line 33:
[[Category: hydrolase(o-glycosyl)]]
[[Category: hydrolase(o-glycosyl)]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:52 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:15:32 2008''

Revision as of 08:15, 20 March 2008

Image:1btc.gif


PDB ID 1btc

Drag the structure with the mouse to rotate
, resolution 2.0Å
Ligands: and
Activity: Beta-amylase, with EC number 3.2.1.2
Coordinates: save as pdb, mmCIF, xml



THREE-DIMENSIONAL STRUCTURE OF SOYBEAN BETA-AMYLASE DETERMINED AT 3.0 ANGSTROMS RESOLUTION: PRELIMINARY CHAIN TRACING OF THE COMPLEX WITH ALPHA-CYCLODEXTRIN


Overview

The three-dimensional structure of a complex of soybean beta-amylase [EC 3.2.1.2] with an inhibitor, alpha-cyclodextrin, has been determined at 3.0 A resolution by X-ray diffraction analysis. Preliminary chain tracing showed that the enzyme folded into large and small domains. The large domain has a (beta alpha)8 super-secondary structure, while the smaller one is formed from two long loops extending from the beta 3 and beta 4 strands of the (beta alpha)8 structure. The interface of the two domains together with shorter loops from the (beta alpha)8 structure form a deep cleft, in which alpha-cyclodextrin binds slightly away from the center. Two maltose molecules also bind in the cleft. One shares a binding site with alpha-cyclodextrin and the other is situated more deeply in the cleft.

About this Structure

1BTC is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of soybean beta-amylase determined at 3.0 A resolution: preliminary chain tracing of the complex with alpha-cyclodextrin., Mikami B, Sato M, Shibata T, Hirose M, Aibara S, Katsube Y, Morita Y, J Biochem. 1992 Oct;112(4):541-6. PMID:1491009

Page seeded by OCA on Thu Mar 20 10:15:32 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1btc"
Personal tools