1bt3
From Proteopedia
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| - | [[Image:1bt3.jpg|left|200px]] | + | [[Image:1bt3.jpg|left|200px]] |
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| - | '''CATECHOL OXIDASE FROM IPOMOEA BATATAS (SWEET POTATOES) IN THE NATIVE CU(II)-CU(II) STATE''' | + | {{Structure |
| + | |PDB= 1bt3 |SIZE=350|CAPTION= <scene name='initialview01'>1bt3</scene>, resolution 2.50Å | ||
| + | |SITE= <scene name='pdbsite=CUA:Binding+Site+For+Cu+A'>CUA</scene> and <scene name='pdbsite=CUB:Binding+Site+For+Cu+B'>CUB</scene> | ||
| + | |LIGAND= <scene name='pdbligand=C2O:CU-O-CU LINKAGE'>C2O</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Catechol_oxidase Catechol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.1 1.10.3.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CATECHOL OXIDASE FROM IPOMOEA BATATAS (SWEET POTATOES) IN THE NATIVE CU(II)-CU(II) STATE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BT3 is a [ | + | 1BT3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ipomoea_batatas Ipomoea batatas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BT3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a plant catechol oxidase containing a dicopper center., Klabunde T, Eicken C, Sacchettini JC, Krebs B, Nat Struct Biol. 1998 Dec;5(12):1084-90. PMID:[http:// | + | Crystal structure of a plant catechol oxidase containing a dicopper center., Klabunde T, Eicken C, Sacchettini JC, Krebs B, Nat Struct Biol. 1998 Dec;5(12):1084-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9846879 9846879] |
[[Category: Catechol oxidase]] | [[Category: Catechol oxidase]] | ||
[[Category: Ipomoea batatas]] | [[Category: Ipomoea batatas]] | ||
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[[Category: catechol oxidase]] | [[Category: catechol oxidase]] | ||
[[Category: dicopper enzyme]] | [[Category: dicopper enzyme]] | ||
| - | [[Category: ipomoea | + | [[Category: ipomoea batata]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:15:25 2008'' |
Revision as of 08:15, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Sites: | and | ||||||
| Ligands: | |||||||
| Activity: | Catechol oxidase, with EC number 1.10.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CATECHOL OXIDASE FROM IPOMOEA BATATAS (SWEET POTATOES) IN THE NATIVE CU(II)-CU(II) STATE
Overview
Catechol oxidases are ubiquitous plant enzymes containing a dinuclear copper center. In the wound-response mechanism of the plant they catalyze the oxidation of a broad range of ortho-diphenols to the corresponding o-quinones coupled with the reduction of oxygen to water. The crystal structures of the enzyme from sweet potato in the resting dicupric Cu(II)-Cu(II) state, the reduced dicuprous Cu(I)-Cu(I) form, and in complex with the inhibitor phenylthiourea were analyzed. The catalytic copper center is accommodated in a central four-helix-bundle located in a hydrophobic pocket close to the surface. Both metal binding sites are composed of three histidine ligands. His 109, ligated to the CuA site, is covalently linked to Cys 92 by an unusual thioether bond. Based on biochemical, spectroscopic and the presented structural data, a catalytical mechanism is proposed in which one of the oxygen atoms of the diphenolic substrate binds to CuB of the oxygenated enzyme.
About this Structure
1BT3 is a Single protein structure of sequence from Ipomoea batatas. Full crystallographic information is available from OCA.
Reference
Crystal structure of a plant catechol oxidase containing a dicopper center., Klabunde T, Eicken C, Sacchettini JC, Krebs B, Nat Struct Biol. 1998 Dec;5(12):1084-90. PMID:9846879
Page seeded by OCA on Thu Mar 20 10:15:25 2008
