1bui
From Proteopedia
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| - | [[Image:1bui.jpg|left|200px]] | + | [[Image:1bui.jpg|left|200px]] |
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| - | '''STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.''' | + | {{Structure |
| + | |PDB= 1bui |SIZE=350|CAPTION= <scene name='initialview01'>1bui</scene>, resolution 2.65Å | ||
| + | |SITE= <scene name='pdbsite=ASA:Catalytic+Site+Of+Chain+A'>ASA</scene> and <scene name='pdbsite=ASB:Catalytic+Site+Of+Chain+B'>ASB</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Plasmin Plasmin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.7 3.4.21.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BUI is a [ | + | 1BUI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUI OCA]. |
==Reference== | ==Reference== | ||
| - | The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action., Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W, Nat Struct Biol. 1998 Oct;5(10):917-23. PMID:[http:// | + | The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action., Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W, Nat Struct Biol. 1998 Oct;5(10):917-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9783753 9783753] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Plasmin]] | [[Category: Plasmin]] | ||
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[[Category: staphylokinase]] | [[Category: staphylokinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:15:58 2008'' |
Revision as of 08:15, 20 March 2008
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| , resolution 2.65Å | |||||||
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| Sites: | and | ||||||
| Activity: | Plasmin, with EC number 3.4.21.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE TERNARY MICROPLASMIN-STAPHYLOKINASE-MICROPLASMIN COMPLEX: A PROTEINASE-COFACTOR-SUBSTRATE COMPLEX IN ACTION.
Contents |
Overview
The serine proteinase plasmin is the key fibrinolytic enzyme that dissolves blood clots and also promotes cell migration and tissue remodeling. Here, we report the 2.65 A crystal structure of a ternary complex of microplasmin-staphylokinase bound to a second microplasmin. The staphylokinase 'cofactor' does not affect the active-site geometry of the plasmin 'enzyme', but instead modifies its subsite specificity by providing additional docking sites for enhanced presentation of the plasminogen 'substrate' to the 'enzymes's' active site. The activation loop of the plasmin 'substrate', cleaved in these crystals, can be reconstructed to show how it runs across the active site of the plasmin 'enzyme' prior to activation cleavage. This is the first experimental structure of a productive proteinase-cofactor-macromolecular substrate complex. Furthermore, it provides a template for the design of improved plasminogen activators and plasmin inhibitors with considerable therapeutical potential.
Disease
Known diseases associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]
About this Structure
1BUI is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The ternary microplasmin-staphylokinase-microplasmin complex is a proteinase-cofactor-substrate complex in action., Parry MA, Fernandez-Catalan C, Bergner A, Huber R, Hopfner KP, Schlott B, Guhrs KH, Bode W, Nat Struct Biol. 1998 Oct;5(10):917-23. PMID:9783753
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