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1bw6
From Proteopedia
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| - | [[Image:1bw6.gif|left|200px]] | + | [[Image:1bw6.gif|left|200px]] |
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| - | '''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | + | {{Structure |
| + | |PDB= 1bw6 |SIZE=350|CAPTION= <scene name='initialview01'>1bw6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1BW6 is a [ | + | 1BW6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW6 OCA]. |
==Reference== | ==Reference== | ||
| - | A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:[http:// | + | A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9451007 9451007] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:16:31 2008'' |
Revision as of 08:16, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1
Overview
CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
About this Structure
1BW6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:9451007
Page seeded by OCA on Thu Mar 20 10:16:31 2008
Categories: Homo sapiens | Single protein | Iwahara, J. | Kigawa, T. | Kitagawa, K. | Masumoto, H. | Okazaki, T. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yokoyama, S. | Centromere protein | Dna-binding | Helix-turn-helix | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
