This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1bb6
From Proteopedia
| Line 23: | Line 23: | ||
[[Category: umbelliferone glycosides]] | [[Category: umbelliferone glycosides]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:53:50 2007'' |
Revision as of 12:49, 30 October 2007
|
LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE
Overview
Two complexes between rainbow trout lysozyme (RBTL) and, 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution., The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that, 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL., This agrees well with earlier crystallographic studies on the binding of, oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to, sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white, lysozymes. For both complexes the 4-MeU moiety in site D has diffuse, electron density and is flexible, as it is only bound to water molecules, and not to the ... [(full description)]
About this Structure
1BB6 is a [Single protein] structure of sequence from [Oncorhynchus mykiss] with UMG as [ligand]. Active as [Lysozyme], with EC number [3.2.1.17]. Structure known Active Site: NUL. Full crystallographic information is available from [OCA].
Reference
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395
Page seeded by OCA on Tue Oct 30 14:53:50 2007
