1c89
From Proteopedia
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| - | [[Image:1c89.gif|left|200px]]< | + | [[Image:1c89.gif|left|200px]] |
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| - | '''NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES''' | + | {{Structure |
| + | |PDB= 1c89 |SIZE=350|CAPTION= <scene name='initialview01'>1c89</scene> | ||
| + | |SITE= <scene name='pdbsite=IC1:Ice+Binding+Site'>IC1</scene> and <scene name='pdbsite=IC2:Ice+Binding+Site'>IC2</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1C89 is a [ | + | 1C89 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]. |
==Reference== | ==Reference== | ||
| - | NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity., Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S, J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:[http:// | + | NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity., Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S, J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11010977 11010977] |
[[Category: Pachycara brachycephalum]] | [[Category: Pachycara brachycephalum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: thermal hysteresis protein]] | [[Category: thermal hysteresis protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:21:07 2008'' |
Revision as of 08:21, 20 March 2008
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NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES
Overview
The structure of a new antifreeze protein (AFP) variant, RD3, from antarctic eel pout (Rhigophila dearborni) with enhanced activity has been determined for the first time by nuclear magnetic resonance spectroscopy. RD3 comprises a unique translational topology of two homologous type III AFP globular domains, each containing one flat, ice binding plane. The ice binding plane of the N domain is located approximately 3.5 A "behind" that of the C domain. The two ice binding planes are located laterally with an angle of 32 +/- 12 degrees between the planes. These results suggest that the C domain plane of RD3 binds first to the ice [1010] prism plane in the <0001> direction, which induces successive ice binding of the N domain in the <0101> direction. This manner of ice binding caused by the unique structural topology of RD3 is thought to be crucial for the significant enhancement of antifreeze activity, especially at low AFP concentrations.
About this Structure
1C89 is a Single protein structure of sequence from Pachycara brachycephalum. Full crystallographic information is available from OCA.
Reference
NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity., Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S, J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977
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