1cgc
From Proteopedia
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| - | [[Image:1cgc.jpg|left|200px]] | + | [[Image:1cgc.jpg|left|200px]] |
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| - | '''DOUBLE HELIX CONFORMATION GROOVE DIMENSIONS AND LIGAND BINDING POTENTIAL OF A G/C-STRETCH IN B-DNA''' | + | {{Structure |
| + | |PDB= 1cgc |SIZE=350|CAPTION= <scene name='initialview01'>1cgc</scene>, resolution 2.200Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''DOUBLE HELIX CONFORMATION GROOVE DIMENSIONS AND LIGAND BINDING POTENTIAL OF A G/C-STRETCH IN B-DNA''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CGC is a [ | + | 1CGC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGC OCA]. |
==Reference== | ==Reference== | ||
| - | Double helix conformation, groove dimensions and ligand binding potential of a G/C stretch in B-DNA., Heinemann U, Alings C, Bansal M, EMBO J. 1992 May;11(5):1931-9. PMID:[http:// | + | Double helix conformation, groove dimensions and ligand binding potential of a G/C stretch in B-DNA., Heinemann U, Alings C, Bansal M, EMBO J. 1992 May;11(5):1931-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1582421 1582421] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bansal, M.]] | [[Category: Bansal, M.]] | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:24:00 2008'' |
Revision as of 08:24, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
DOUBLE HELIX CONFORMATION GROOVE DIMENSIONS AND LIGAND BINDING POTENTIAL OF A G/C-STRETCH IN B-DNA
Overview
The self-complementary DNA fragment CCGGCGCCGG crystallizes in the rhombohedral space group R3 with unit cell parameters a = 54.07 A and c = 44.59 A. The structure has been determined by X-ray diffraction methods at 2.2 A resolution and refined to an R value of 16.7%. In the crystal, the decamer forms B-DNA double helices with characteristic groove dimensions: compared with B-DNA of random sequence, the minor groove is wide and deep and the major groove is rather shallow. Local base pair geometries and stacking patterns are within the range commonly observed in B-DNA crystal structures. The duplex bears no resemblance to A-form DNA as might have been expected for a sequence with only GC base pairs. The shallow major groove permits an unusual crystal packing pattern with several direct intermolecular hydrogen bonds between phosphate oxygens and cytosine amino groups. In addition, decameric duplexes form quasi-infinite double helices in the crystal by end-to-end stacking. The groove geometries and accessibilities of this molecule as observed in the crystal may be important for the mode of binding of both proteins and drug molecules to G/C stretches in DNA.
About this Structure
1CGC is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Double helix conformation, groove dimensions and ligand binding potential of a G/C stretch in B-DNA., Heinemann U, Alings C, Bansal M, EMBO J. 1992 May;11(5):1931-9. PMID:1582421
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