1cgp
From Proteopedia
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| - | [[Image:1cgp.gif|left|200px]] | + | [[Image:1cgp.gif|left|200px]] |
| - | + | ||
| - | '''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE''' | + | {{Structure |
| + | |PDB= 1cgp |SIZE=350|CAPTION= <scene name='initialview01'>1cgp</scene>, resolution 3.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CMP:ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'>CMP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CGP is a [ | + | 1CGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1CGP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGP OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:[http:// | + | Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1653449 1653449] |
[[Category: Catabolite Activator Protein]] | [[Category: Catabolite Activator Protein]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:24:09 2008'' |
Revision as of 08:24, 20 March 2008
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| , resolution 3.000Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Overview
The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90 degrees. This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor--DNA complex provides insights into possible mechanisms of transcription activation.
About this Structure
1CGP is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1CGP with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:1653449
Page seeded by OCA on Thu Mar 20 10:24:09 2008
