1ift

From Proteopedia

Revision as of 13:34, 30 October 2007

RICIN A-CHAIN (RECOMBINANT)

Overview

Ricin is a potent plant toxin which acts by removing a specific adenine, residue from the ribosome. The X-ray crystal structure of a new, tetragonal crystal form of the recombinant ricin A-chain diffracting to, 1.8 A resolution has been determined via molecular replacement methods and, refined to a crystallographic R-factor of 18.6%. The higher resolution, electron density allowed improvements to be made upon previously published, models, resulting in an increase in the assigned secondary structure of, the protein. The enzyme adopts the same global conformation in this, crystal form with differences in detail due only partly to crystal, packing. The active site superimposes closely with those of previously, published models but the locations of the active-site water molecules, differ in ... [(full description)]

About this Structure

1IFT is a [Single protein] structure of sequence from [Ricinus communis]. Active as [rRNA N-glycosylase], with EC number [3.2.2.22]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].

Reference

X-ray structure of recombinant ricin A-chain at 1.8 A resolution., Weston SA, Tucker AD, Thatcher DR, Derbyshire DJ, Pauptit RA, J Mol Biol. 1994 Dec 9;244(4):410-22. PMID:7990130

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