2j4f

From Proteopedia

Revision as of 15:19, 30 October 2007

TORPEDO ACETYLCHOLINESTERASE- HG HEAVY-ATOM DERIVATIVE

Overview

Chemical modification of Torpedo californica acetylcholinesterase by, various sulfhydryl reagents results in its conversion to one of two, principal states. One of these states, viz., that produced by disulfides, and alkylating agents, is stable. The second state, produced by mercury, derivatives, is metastable. At room temperature, it converts, spontaneously, with a half-life of ca. 1 h, to a stable state similar to, that produced by the disulfides and alkylating agents. Demodification of, acetylcholinesterase freshly modified by mercurials, by its exposure to, reduced glutathione, causes rapid release of the bound mercurial, with, concomitant recovery of most of the enzymic activity of the native enzyme., In contrast, similar demodification of acetylcholinesterase modified by, ... [(full description)]

About this Structure

2J4F is a [Single protein] structure of sequence from [Torpedo californica] with HG as [ligand]. Active as [Acetylcholinesterase], with EC number [3.1.1.7]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

A metastable state of Torpedo californica acetylcholinesterase generated by modification with organomercurials., Kreimer DI, Dolginova EA, Raves M, Sussman JL, Silman I, Weiner L, Biochemistry. 1994 Dec 6;33(48):14407-18. PMID:7981200

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