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1clk

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[[Image:1clk.gif|left|200px]]<br /><applet load="1clk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1clk.gif|left|200px]]
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caption="1clk, resolution 1.90&Aring;" />
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'''CRYSTAL STRUCTURE OF STREPTOMYCES DIASTATICUS NO.7 STRAIN M1033 XYLOSE ISOMERASE AT 1.9 A RESOLUTION WITH PSEUDO-I222 SPACE GROUP'''<br />
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{{Structure
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|PDB= 1clk |SIZE=350|CAPTION= <scene name='initialview01'>1clk</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5]
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF STREPTOMYCES DIASTATICUS NO.7 STRAIN M1033 XYLOSE ISOMERASE AT 1.9 A RESOLUTION WITH PSEUDO-I222 SPACE GROUP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1CLK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_diastaticus Streptomyces diastaticus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLK OCA].
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1CLK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_diastaticus Streptomyces diastaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLK OCA].
==Reference==
==Reference==
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Structure of xylose isomerase from Streptomyces diastaticus no. 7 strain M1033 at 1.85 A resolution., Zhu X, Teng M, Niu L, Xu C, Wang Y, Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):129-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10666592 10666592]
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Structure of xylose isomerase from Streptomyces diastaticus no. 7 strain M1033 at 1.85 A resolution., Zhu X, Teng M, Niu L, Xu C, Wang Y, Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):129-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10666592 10666592]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces diastaticus]]
[[Category: Streptomyces diastaticus]]
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[[Category: isomerase]]
[[Category: isomerase]]
[[Category: pseudo-i222]]
[[Category: pseudo-i222]]
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[[Category: streptomyces]]
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[[Category: streptomyce]]
[[Category: xylose isomerase]]
[[Category: xylose isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:07:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:25:54 2008''

Revision as of 08:25, 20 March 2008

Image:1clk.gif


PDB ID 1clk

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands: and
Activity: Xylose isomerase, with EC number 5.3.1.5
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF STREPTOMYCES DIASTATICUS NO.7 STRAIN M1033 XYLOSE ISOMERASE AT 1.9 A RESOLUTION WITH PSEUDO-I222 SPACE GROUP


Overview

The structure of xylose isomerase (XyI) from Streptomyces diastaticus No. 7 strain M1033 (SDXyI) has been refined at 1.85 A resolution to conventional and free R factors of 0.166 and 0.219, respectively. SDXyI was crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 87.976, b = 98.836, c = 93.927 A. One dimer of the tetrametric molecule is found in each asymmetric unit. Each monomer consists of two domains: a large N-terminal domain (residues 1-320), containing a parallel eight-stranded alpha/beta barrel, and a small C-terminal loop (residues 321-387), containing five helices linked by random coil. The four monomers are essentially identical in the tetramer, possessing non-crystallographic 222 symmetry with one twofold axis essentially coincident with the crystallographic twofold axis in the space group P2(1)2(1)2, which may explain why the diffraction pattern has strong pseudo-I222 symmetry even at medium resolution. The crystal structures of XyIs from different bacterial strains, especially from Streptomyces, are similar. The alpha2 helix of the alpha/beta barrel has a different position in the structures of different XyIs. The conformation of C-terminal fragment 357-364 in the SDXyI structure has a small number of differences to that of other XyIs. Two Co(2+) ions rather than Mg(2+) ions exist in the active site of the SDXyI structure; SDXyI seems to prefer to bind Co(2+) ions rather than Mg(2+) ions.

About this Structure

1CLK is a Single protein structure of sequence from Streptomyces diastaticus. Full crystallographic information is available from OCA.

Reference

Structure of xylose isomerase from Streptomyces diastaticus no. 7 strain M1033 at 1.85 A resolution., Zhu X, Teng M, Niu L, Xu C, Wang Y, Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):129-36. PMID:10666592

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