1cq2
From Proteopedia
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| - | [[Image:1cq2.gif|left|200px]] | + | [[Image:1cq2.gif|left|200px]] |
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| - | '''NEUTRON STRUTURE OF FULLY DEUTERATED SPERM WHALE MYOGLOBIN AT 2.0 ANGSTROM''' | + | {{Structure |
| + | |PDB= 1cq2 |SIZE=350|CAPTION= <scene name='initialview01'>1cq2</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NEUTRON STRUTURE OF FULLY DEUTERATED SPERM WHALE MYOGLOBIN AT 2.0 ANGSTROM''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CQ2 is a [ | + | 1CQ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQ2 OCA]. |
==Reference== | ==Reference== | ||
| - | Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin., Shu F, Ramakrishnan V, Schoenborn BP, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3872-7. PMID:[http:// | + | Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin., Shu F, Ramakrishnan V, Schoenborn BP, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3872-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10725379 10725379] |
[[Category: Physeter catodon]] | [[Category: Physeter catodon]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: helical]] | [[Category: helical]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:27:27 2008'' |
Revision as of 08:27, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NEUTRON STRUTURE OF FULLY DEUTERATED SPERM WHALE MYOGLOBIN AT 2.0 ANGSTROM
Overview
Although hydrogens comprise half of the atoms in a protein molecule and are of great importance chemically and structurally, direct visualization of them by using crystallography is difficult. Neutron crystallography is capable of directly revealing the position of hydrogens, but its use on unlabeled samples faces certain technical difficulties: the large incoherent scattering of hydrogen results in background scattering that greatly reduces the signal to noise of the experiment. Moreover, whereas the scattering lengths of C, N, and O are positive, that of hydrogen is negative and about half the magnitude. This results in density for hydrogens being half as strong and close to the threshold of detection at 2.0-A resolution. Also, because of its opposite sign, there is a partial cancellation of the hydrogen density with that from neighboring atoms, which can lead to ambiguities in interpretation at medium resolution. These difficulties can be overcome by the use of deuterated protein, and we present here a neutron structure of fully deuterated myoglobin. The structure reveals a wealth of chemical information about the molecule, including the geometry of hydrogen bonding, states of protonation of histidines, and the location and geometry of water molecules at the surface of the protein. The structure also should be of broader interest because it will serve as a benchmark for molecular dynamics and energy minimization calculations and for comparison with NMR studies.
About this Structure
1CQ2 is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.
Reference
Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin., Shu F, Ramakrishnan V, Schoenborn BP, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3872-7. PMID:10725379
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